卡马西平对大鼠海马脑片诱发场电位抑制作用的可能机制  

Inhibition of field potentials recorded from rat hippocampus slices by carbamazepine might be achieved through endogenous GABA

作　　者：张敬东[1] 佟振清 斋藤喜八[2] 

机构地区：[1]第一军医大学生理学教研室,广州市510515 [2]大阪大学齿学部药理学

出　　处：《第一军医大学学报》1997年第3期185-188,共4页Journal of First Military Medical University

摘　　要：抑制剂量的卡马西平（CBZ）可抑制大鼠海马脑片的场电位使其幅值显著减小。非抑制剂量的CBZ（小于等于2×10-4mol／L）与γ-氨基丁酸（GABA.10－4，2×10－4，5×10－4和10－3mol／L）合用，抑制场电位的作用比单用GABA显著增强。用β-疏基丙酸10－4mol／L预处理脑片40－60min降低内源性GABA产量后，场电位幅值稳定地增大40％左右．此后再给抑制剂量的CBZ（3×10－4mol／L）对场电位无明显抑制作用，而CBZ与GABA合用则又显示了显著的抑制作用．上述结果表明，CBZ抑制场电位的作用可能是通过内源性GABA实现的。Carbamazepine (CBZ) 10-4and 2×10-4mol/L did not inhibitively affect the field potentials recorded from CA1 pyrarnidal cells,while CBZ 3 × 10-4 and 5-10-4mol/L distinctly depressed the amplitt1de of the field potentials. However, CBZ 2×10-4 mol/L together with GABA (10-4, 2 × 10-4, 5 ×10-4and 10-3 mol/L )brought about significantly stronger inhibition on the field potentials than that of GABA (same concentrations) alone. β-mercaptopropionic acid (MPA) 10-4 mol/L was used to preincubate hippocampus slices 40-60min,which could decrease the production of endogenous GABA and increase the amplittlde of field potentials by about 40%. If CBZ 3 × 10-4 mol/L was given after MPA, no distinct inhibition was observed; while this CBZ together with GABA (3 ×10-4mol/L) brought about strong inhibition. CBZ 5 ×10-4mol/L resulted in a similar outcome. The present results indicated that non-inhibitory dose of CBZ could facilitate the inhibitory effects of GABA on field potentials recorded from hippocampus slices, and inhibitory dose of CBZ might achieve its inhibition through endogenous GABA.

关 键 词：卡马西平 海马 场电位 抑制 GABA MPA 

分 类 号：R338.26[医药卫生—人体生理学] R971.6[医药卫生—基础医学]