卢帕他定对大鼠油酸型急性肺损伤的保护作用研究  

Effect of Rupatadine on Acute Lung Injury Induced by Oleic Acid in Rats

作　　者：张林丽[1] 何建林[1] 余书勤[1] 

机构地区：[1]南京师范大学生命科学学院新药研究中心,江苏省医学分子生物学重点实验室,南京210046

出　　处：《中国药学杂志》2008年第6期420-423,443,共5页Chinese Pharmaceutical Journal

基　　金：江苏省高校自然科学重大基础研究资助(2005104TSJ0116)

摘　　要：目的探讨卢帕他定(rupatadine)对大鼠油酸型急性肺损伤(acute lung injury,ALI)的作用特性及其对肿瘤坏死因子-α(TNF-α)表达的影响。方法制备油酸型大鼠急性肺损伤模型,利用动物肺功能测定仪测定肺损伤过程中吸气气道阻力(IR)、呼气气道阻力(ER)和动态肺顺应性(Cdyn)的变化并分析诸项指标变化的意义、称重法计算湿/干重比(W/D),紫外分光光度法检测肺泡灌洗液(BALF)中蛋白含量,酶联免疫吸附法(ELISA)检测TNF-α含量。结果卢帕他定可显著改善油酸型肺损伤大鼠的肺功能指标,降低肺组织湿/干重比,减少肺泡灌冼液中蛋白含量,抑制TNF-α的升高,抑制率达到24.22%。结论卢帕他定时大鼠油酸型肺损伤有明显的保护作用且具剂量依赖性。OBJECTIVE To investigate the either of rupatadine on acute lung injury induced by oleic acid in rats. METHODS Rats were randomly divided into six groups. The changes of lung inspiratory resistance,expiratory resistance and dynamic lung compliance were recorded. BALF （bronehnalveolar lavage fluid） protein was measured by spectrophntometer and TNF-α level was examined by Enzyme linked immunosorbent assay （ELISA）. Lung wet to dry weight ratio was calculaled to assess alveolar edema. RESULTS The pretreatment of rupatadiue significantly alleviated oleic aeid-induced lung injury accompanied by the improvement of lung function and reduction of lung wet to dry weight ratio. Funrthenuore, rupatadine also decreased the concentration of protein in BALF and inhibited the increase of the TNF-α significantly. CONCLUSION Rnpatadine has a dose-dependent beneficial effect on acute lung injury induced by oleie acid in rats. The uhimate reduction of inflamunatory factor was involved,at least in part,in the mechanism of rupatadine effects.

关 键 词：卢帕他定 油酸 急性肺损伤 肺功能 肿瘤坏死因子-Α 

分 类 号：R965[医药卫生—药理学]