槲皮素固体脂质纳米粒的制备及小鼠口服吸收研究  

Studies on Preparation of Quercetin Solid Lipid Nanoparticles and Oral Absorption in Mice

作　　者：李厚丽[1] 翟光喜[1] 祝伟伟[1] 李凌冰[1] 马玉坤[2] 

机构地区：[1]山东大学药学院,济南250012 [2]济南市第二人民医院药剂科,济南250005

出　　处：《中国药学杂志》2008年第6期435-438,共4页Chinese Pharmaceutical Journal

基　　金：山东省优秀中青年科学家奖励基金课题(2004BS03019)

摘　　要：目的制备槲皮素固体脂质纳米粒(QT-SLN),并考察其理化性质及小鼠口服吸收特性。方法以具有生物相容性的硬脂酸作为脂质载体,采用高温乳化-低温固化法制备槲皮素固体脂质纳米粒,以均匀设计法优化处方与制备工艺;透射电镜(TEM)观察其微观形态,并测定其粒径分布;建立测定纳米粒和胃肠道内容物及粪样混合物中槲皮素的紫外分光光度法。结果槲皮素固体脂质纳米粒为球状或类球状,平均粒径为217.3 nm,包封率为48.50%,其小鼠体内吸收优于槲皮素原料药。结论高温乳化-低温固化法适于制备槲皮素固体脂质纳米粒,该制剂促进了槲皮素的小鼠口服吸收。OBJECTIVE To prepare quercetin-loaded solid lipid nanoparticles （QT-SLN） and investigate its properties and the oral absorption character in mice, METHODS QT-SLN was prepared by the method of emulsion evaporation at a high temperature and solidification at a low temperature. Stearic acid with high biocompatibility was used as lipid carrier. The optimum formulation was selected by uniform design, Its morphology was examined by transmission electron microscope（TEM） and the particle diameter distribution was decided. A UV-vis analytical method for the determination of quercetin in the nanoparticles and in the mixture of gastrointestinal contents and feces was established. RESULTS QT-SLN was spherical shape with mean diameter of 217. 3 nm and entrapment efficiency （EE） of 48. 50%. The oral absorption of QT-SLN in mice was better than that of quercetin suspension. CONCLUSION QT-SI,N can be prepared by the method of emulsion evaporation at a high temperature and solidification at a low temperature,and it also can improve the oral absorption of qucrcetin in mice.

关 键 词：槲皮素 固体脂质纳米粒 均匀设计 口服吸收 

分 类 号：R944[医药卫生—药剂学]