黄瓜与酸黄瓜异源多倍体3种同工酶表达基因组剂量效应  

Genome Dosage Effect on Three Kinds of Isozyme Expression Patterns in Cucumis Allopolyploid

作　　者：罗向东[1,2] 戴亮芳[1,2] 钱春桃[1] 娄群峰[1] 陈劲枫[1] 

机构地区：[1]作物遗传与种质创新国家重点实验室,南京农业大学园艺学院,南京210095 [2]江西师范大学生命科学学院,南昌330022

出　　处：《园艺学报》2008年第3期377-382,共6页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30470120);国家‘863’计划项目(2006AA10Z1A8,2006AA100108);国家科技支撑计划项目(2006BAD13B06);江苏省高新技术项目(2007);国际腌渍协会腌渍种子研究基金项目~~

摘　　要：以甜瓜属(Cucumis)黄瓜与酸黄瓜的3种不同倍性异源多倍体—双单倍体种间杂种F1(2n=2x=19,HC)、异源三倍体黄瓜(2n=3x=26,HCC)和异源四倍体C.hytivus(2n=4x=38,HHCC)及其双亲为试材,比较研究过氧化物酶(POD)、谷氨酸脱氢酶(GDH)和天冬氨酸转氨酶(AAT)在不同倍性异源多倍体中的酶谱表达特性,探讨基因组剂量对酶谱表达的影响。结果表明:不同倍性异源多倍体的酶带染色力表现出明显的差异,但酶带数和酶带分布基本一致。在AAT同工酶中,酶带的表达随基因组剂量的提高而增强,在POD和GDH同工酶中,大部分酶带的表达随基因组剂量的提高而减弱。这些结果表明甜瓜属异源多倍体在不同的酶系统中,酶谱表达可能会表现出截然相反的基因组剂量效应。本研究结果可为鉴定甜瓜属异源多倍体和阐述异源多倍体因倍性引起的表型差异提供同工酶证据。Three alloploids with different ploidies from interspecific hybridization in Cucumis [ interspecific hybrids （2n =2x = 19, HC）, allotriploid （2n =3x =26, HCC）, and allotetraploid C. hytivus （2n =4x = 38, HHCC） ] and their parents were used for the comparative studies on expression pattern of peroxidase （POD）, glutamate dehydrogenase （GDH）, and aspartate aminotransferase （AAT）. The results showed that the zymograms in the alloploids were similar in band number and band distribution. The intensity of band in these allopolyploids, however, presents significant difference, which was caused by genome dosage. In AAT zymogram, the intensity of band was positively correlated with ploidy level. In POD and GDH zymogram, however, the intensity of band was negatively correlated with ploidy level. It was indicated that the coherence between genome dosage and isozyme expression pattern would be opposite in different enzyme systems. These data will be helpful to illuminate the morphological changes caused by the polyploid level.

关 键 词：甜瓜属 黄瓜 同工酶 基因组剂量 异源多倍体 

分 类 号：S642.2[农业科学—蔬菜学]