平邑甜茶叶片不定芽再生及NO的效应  

Adventitious Shoot Regeneration from Leaves of Malus hupehensis and Effects of Nitric Oxide

作　　者：韩小娇[1] 杨洪强[1] 由淑贞[1] 段凯旋[1] 张鑫荣[1] 赵海洲[1] 

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018

出　　处：《园艺学报》2008年第3期419-422,共4页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30671452;30571285);高校博士点科研基金项目(20050434009)

摘　　要：以平邑甜茶继代组培苗叶片为材料,研究了一氧化氮(NO)供体——硝普钠(SNP)、6-BA、NAA、2,4-D、ZT和光暗条件对平邑甜茶叶片不定芽再生的影响。结果表明,6-BA、ZT、NAA和暗培养均有利于叶片不定芽的分化;在含有生长素和细胞分裂素的MS培养基中,加入6.0和12.0mg·L-1的SNP可极显著诱导叶片不定芽的分化;以MS+6.0mg·L-1BA+0.5mg·L-1NAA+0.2mg·L-1ZT+6.0mg·L-1SNP为培养基,暗培养15d,叶片不定芽分化效果最好,再生率达67.8%。The effects of nitric oxide donor-sodium nitroprusside （SNP）, 6-BA, NAA, 2,4-D, ZT and dark treatment on adventitious shoot regeneration from leaves of Malus hupehensis Rehd. were studied. The resuits showed that 6-BA, ZT, NAA and dark treatment could increase adventitious shoot differentiation. In the presence of auxin and cytokinin, 6. 0 and 12. 0 mg· L^-1SNP could significantly induce leaf adventitious shoot differentiation. The best result was achieved in the medium MS ＋ 6. 0 mg ·L^-1 BA ＋ 0. 5 mg · L^-1 NAA ＋ 0. 2 mg · L^-1 ZT ＋ 6. 0 mg· L^-1 SNP, in which the percentage of adventitious shoot regeneration were 67.8% after dark treatment for 15 d.

关 键 词：平邑甜茶 叶片 不定芽再生 一氧化氮 

分 类 号：S661[农业科学—果树学]