适配子型压电石英晶体传感器检测人免疫球蛋白E的实验研究  

Piezoelectric Immunoglobulin Aptamer Biosensor for Determination of IgE

作　　者：姚春艳[1] 齐永志[1] 府伟灵[1] 

机构地区：[1]第三军医大学西南医院检验科,重庆400038

出　　处：《中华医院感染学杂志》2008年第3期324-327,共4页Chinese Journal of Nosocomiology

基　　金：国家科技部“九五”科学仪器攻关项目(96-A23-04-04);国家高技术生物技术领域(863);青年基金(2002AARZ2003);国家自然科学基金(30400420);军队“十五”课题资助(01MA18001LO065)

摘　　要：目的研制适配子型压电子石英晶体传感器阵列与常用的免疫分析方法进行比较,以评价压电石英晶体传感器阵列检测灵敏度、特异性与临床检测的可行性。方法以AT切型、基频10MHz的镀金石英晶体作为换能器,将抗人免疫球蛋白E适配子固定在石英晶体电极表面,用2×5检测池阵列构建新型适配子型压电石英晶体传感器;分别进行标准品及人血清免疫球蛋白E的检测,研究了检测灵敏度、特异性等检测指标。结果该传感器可快速完成检测且无非特异性反应,在检测范围内传感器的响应特性良好,压电晶体频率偏移值与免疫球蛋白浓度呈良好的线性关系;将此免疫传感器用于人血清标本的测定,结果与化学发光法符合(r值=0.9924)。结论研制的传感器阵列具有灵敏度高、特异性好,检测通量高,无需标记,操作简便,能实时在线检测等优点,可用于临床实验诊断,具有较高的临床推广应用价值。OBJECTIVE A new piezoelectric aptamer biosensor is developed for determination of IgE. The energy converters are 10MHz AT-cut quartz crystals with gold-coated electrodes. The anti-IgE aptamers are immobilized onto the surfaces of crystals gold electrodes by biotin-avidin method. METHODS The standard substance and serum were detected to find the limit of detection and specificity of the biosensor. RESULTS The piezoelectric immunoglobulin aptamer biosensor could complete the detection without nonspecific response. Under the optimized conditions, the experimental results showed that the piezoelectric biosensor had good response to IgE whose frequency shifts were linearly dependent on IgE concentration in different range. The piezoelectric aptamer biosensor had been used to detect IgE in serum, the analytical results given by this method were in satisfactory agreement with those given by chemoluminescence method, its correlation coefficient was 0. 9924. CONCLUSIONS Piezoelectric aptamer biosensor for the determination of IgE is of high sensitivity, high specificity, high analysis speed, unnecessary labeling, simple operation, real-time detection, etc. It is suitable for detection of IgE and should be used for clinical detection.

关 键 词：压电石英晶体传感器 阵列 适配子 免疫球蛋白 

分 类 号：R446[医药卫生—诊断学]