印迹基因H19对人绒毛膜上皮癌细胞JEG-3侵袭能力的影响  

Effect of Imprinting Gene H19 on Invasion Ability of Human Choriocarcinoma Cell Line JEG-3

作　　者：俞丽丽[1] 李力[1] 陈鸣[2] 张伟[3] 卢林杉[1] 赵丹[1] 陈星云[4] 李平[4] 周元国[4] 

机构地区：[1]第三军医大学大坪医院妇产科,重庆400042 [2]第三军医大学西南医院检验科,重庆400038 [3]第三军医大学劳动卫生教研室,重庆400038 [4]第三军医大学野战外科研究所七室,重庆400042

出　　处：《中华医院感染学杂志》2008年第3期332-335,共4页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金(30500542)

摘　　要：目的探讨印迹基因H19对滋养细胞侵袭能力的影响,以期从分子遗传学角度来阐明滋养细胞侵袭行为的调控机制。方法含人全长H19 cDNA重组真核表达质粒pRc/CMV经测序鉴定正确后,转染滋养细胞株JEG-3,观察其H19 mRNA表达和细胞侵袭力的变化。结果质粒转染JEG-3细胞的转染效率>50%,转染目的基因H19后mRNA表达较对照组显著升高;转染目的基因后JEG-3细胞穿膜细胞数明显少于未转染组和空载体转染组。结论H19基因过表达可抑制JEG-3的侵袭能力,为H19调控滋养细胞侵袭行为提供了直接的实验证据。OBJECTIVE To investigate the effect of imprinting gene H19 on the invasion ability of trophoblast cell in order to explore the regulatory mechanism of the invasion behavior of trophoblast cell from molecular genetics. METHODS After correct identification with sequencing for the recombinant eukaryotie expression plasmid pRe/ CMV including the whole length of H19 eDNA, the plasmid was transfected to the cell line JEG-3. The expression of H19 mRNA and the change in cellular invasion ability were observed. RESULTS The transfeetion efficiency for the plasmid was higher than 50%. And after being transfected with target H19 gene, the expression of the mRNA level was significantly increased compared with that of control group. And the cell numbers invading through the matrigel filter were significantly decreased compared with that of untransfeetion and empty carrier groups. CONCLUSIONS Over-expression of H19 gene can suppress trophoblast cell invasion in vitro. It provides the direct experimental evidence for regulating the cellular invasion behavior with H19 gene.

关 键 词：H19基因 JEG-3细胞 侵袭 

分 类 号：R174.12[医药卫生—妇幼卫生保健] R739.6[医药卫生—公共卫生与预防医学]