探针浓度值对LSAW-bisPNA传感器检测系统的影响  

Effects of Probe Concentrations on LSAW-bisPNA Gene Sensor Detection System

作　　者：陈鸣[1] 张烨[1] 王云霞[1] 丁毅[2] 曹亮[2] 府伟灵[1] 

机构地区：[1]第三军医大学西南医院检验科,重庆400038 [2]中国电子科技集团公司第二十六研究所第五研究室,重庆400060

出　　处：《中华医院感染学杂志》2008年第3期342-344,共3页Chinese Journal of Nosocomiology

基　　金：国家863计划重大专项(2002AARZ2023);国家863探索导向性课题(2007AA02Z416);国家自然科学基金(30400107);军队“十一五”课题(06G073);重庆市科技攻关(8327)

摘　　要：目的探讨双体肽核酸(bisPNA)探针浓度值对漏声表面波(LSAW)-bisPNA基因传感器检测系统的影响。方法LSAW基因传感器表面分别固定终浓度为0.001、0.005、0.01、0.05、0.5、1.0、1.5、2.0、3.0、4.0μmol/L的bisPNA探针后,分别观察其与相应的HPV靶序列杂交时相位变化及反应所需时间。结果与HPV靶序列杂交所引起的相位变化值是先升后降,以1.0μmol/L探针浓度为分界线;杂交平衡时间上各组间未见明显的变化趋势。结论1.0μmol/L bisPNA探针固定浓度最为适宜。OBJECTIVE To investigate the effects of bis-peptide nucleic acid （bisPNA） probe concentrations on hybridization efficiency with leaky surface acoustic wave （LSAW）-bisPNA gene sensor detection system. METHODS Different concentrations of bisPNA probe varied from 0. 001 to 4. 0 μmol/L were respectively immobilized on the surface of LSAW gene sensor. Then it was hybridized with corresponding HPV target sequence. And then the change in phase and equilibrium time of reaction was recorded and analyzed. RESULTS When bisPNA probe concentration varied from 0. 001 to 4.0 μmol/L, the change in phase resulted in hybridization reaction increased firstly and then decreased, and 1. 0 μmol/L was the watershed. And change tendency of equilibrium time was not obvious. CONCLUSIONS Optimal immobilizing probe concentration is 1.0 μmol/L.

关 键 词：肽核酸 探针 漏声表面波 基因传感器 杂交 

分 类 号：R446[医药卫生—诊断学]