转铁蛋白受体介导的Survivin反义RNA基因转移系统的构建及体外效应研究  

Establishment and in Vitro Biological Effect of Survivin Antisense RNA Transfer System Mediated by Transferrin Receptor

作　　者：佟豪[1] 巴雅尔[2] 王晓娟[1] 

机构地区：[1]内蒙古医学院基础学院免疫教研室,内蒙古呼和浩特010059 [2]内蒙古医学院第三附属医院普外科,内蒙古包头014010

出　　处：《内蒙古医学杂志》2008年第2期142-144,F0004,共4页Inner Mongolia Medical Journal

摘　　要：目的:构建转铁蛋白受体介导的Survivin反义RNA基因转移系统并观察其对肝癌细胞的体外杀伤效应及凋亡检测。方法:用EDC法制备抗转铁蛋白受体与多聚左旋赖氨酸(PLL)的复合物并用分子筛层析纯化。根据DNA阻滞实验结果Survivin反义RNA重组质粒与Ab-PLL按1:3混合使二者结合形成Ab-pLL-Survivin反义RNA(质粒DNA)复合物,将此复合物转染人肝癌细胞株HepG2细胞观察其对HepG2细胞的杀伤效应及细胞凋亡的影响。结果:转染复合物组HepG2细胞的增殖受抑制、凋亡明显增多。结论:本基因体系具有很好的靶向性及杀伤、促凋亡效应。Objective： To establish the surviving by transferring receptor, and to observe its special lethal effect apoptosis - induced effect on liver cancer cell. Methods ： The conjugate of anti - TfR McAb and poly - L - lysine（PLL） was made by EDC and purified by molecular screen chromatography. According to the results of DNA block experiment, antisense surviving cDNA recombinant plasmid and McAb - PLL were combined by the ratio of 1：3, then the antisense survivin recombinant/McAb- PLL complex was used to transfer human hepatoma cell line HepG2, The lethal and apoptosis effects on HepG2 cell were observed. Results： HepG2 cellular proliferation was suppressed and its apoptosis increased in group with transduced complex. Conclusion： The gene system has better effect on targetly killing and improving apoptosis.

关 键 词：靶向性 基因转移系统 肝癌 基因治疗 

分 类 号：R703.3[医药卫生—临床医学]