加拿大披碱草×肥披碱草杂种F_1幼穗组培再生体系的研究  

Studies on the Tissue Culture and Regeneration System of Hybrid F_1 of Elymus canadensis×E. excelsus

作　　者：李小雷[1] 于卓[1] 马艳红[1] 谢芳利[1] 李造哲[1] 周亚星[1] 

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010019

出　　处：《安徽农业科学》2008年第6期2235-2237,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30560100);内蒙古自然科学基金项目(200607010505)

摘　　要：[目的]筛选适宜加拿大披碱草×肥披碱草种间杂种F1愈伤组织诱导、幼苗分化和生根的最适培养基。[方法]以加拿大披碱草×肥披碱草种间杂种F1幼穗为外植体,以MS为基本培养基,添加不同的激素和营养物,探讨了2,4-D、6-BA、NAA与IBA4种激素不同浓度对杂种F1愈伤组织的诱导、分化及生根的影响,建立了组培再生体系。[结果]激素2,4-D对杂种F1愈伤组织诱导起决定性作用。适宜杂种F1愈伤组织诱导的最佳培养基为:MS+CH300.0 mg/L+2,4-D2.0 mg/L,诱导率达100%;适宜幼苗分化的最佳培养基为:MS+CH300.0 mg/L+6-BA2.0 mg/L+NAA0.4 mg/L,分化率达73.3%;适宜生根的最佳培养基为:MS+CH300.0 mg/L+IBA0.5 mg/L,生根率达86.7%;组培苗经过炼苗移栽得到了完整植株。[结论]杂种F1组培再生体系的建立为新种质的保存奠定了基础。2,4-D适宜浓度为2.0 mg/L。[Objective] The aim of research was to screen the optimal medium of callus induction,plantlets differentiation and rooting of hybrid F1 of Elymus Canadensis×E.excelsus.[Method] With the young spikes of hybrid F1 of E.Canadensis×E.excelsus as explant and MS as basic medium,the different hormones and nutrients were added to the MS medium to discuss the effect of 4 kinds of hormones including 2,4-D,6-BA,NAA and IBA.on the callus induction,plantlet differentiation and rooting of hybrid F1 and the plant regeneration system was established.[Result] The hormone 2,4-D played a decisive role in the callus-inducing of hybrid F1.The optimal medium of callus induction of hybrid F1 was MS ＋ CH 300.0 mg/L ＋ 2,4-D 2.0 mg/L with the induction rate of 100%.The optimal medium of plantlets differentiation was MS ＋ CH 300.0 mg/L ＋6-BA 2.0 mg/L ＋ NAA 0.4 mg/L with the differentiation rate of 73.3%.The optimal medium of rooting was MS ＋ CH 300.0 mg/L＋IBA 0.5 mg/L with the rooting rate of 86.7%,and the regenerated plant was ob tained after acclimatization and transplanting.[Conclusion] The establishment of tissue culture and regeneration system laid a foundation for preservation of germplasm resource.

关 键 词：加拿大披碱草 肥披碱草 种间杂种F1 愈伤组织诱导 植株再生 

分 类 号：Q343.244[生物学—遗传学]