B7-H1及其受体PD-1在胃癌组织中的表达与意义  

Expression and significance of B7-H1 and its receptor PD-1 in human gastric carcinoma

作　　者：刘书漫[1] 孟青[1] 张钦宪[1] 王盛典[2] 刘占举[3] 张谢夫[4] 

机构地区：[1]郑州大学基础医学院组胚教研室,450052 [2]中国科学院生物物理研究所大分子国家重点实验室感染与免疫中心 [3]郑州大学第二附属医院消化内科免疫病理实验室 [4]郑州大学第一附属医院普外科

出　　处：《中华肿瘤杂志》2008年第3期192-195,共4页Chinese Journal of Oncology

基　　金：教育部新世纪优秀人才支持计划（NECT-05-0609）;河南省重大科技攻关项目（0222031300）

摘　　要：目的探讨B7-H1和PD-1信号通路与胃癌发生的关系。方法利用免疫组化技术和ANAE染色技术检测胃组织中B7-H1和PD-1的原位表达及肿瘤浸润淋巴细胞（TIL）浸润情况，利用逆转录聚合酶链反应（RT-PCR）技术检测胃癌组织中B7-H1和PD-1 mRNA的表达，利用Western blot技术检测B7-H1蛋白的表达。结果B7-H1和PD-1在胃癌组织中表达升高，阳性率分别为64．4％和43．8％，而在正常胃组织中不表达。胃癌组织中，B7-H1表达与TIL浸润负相关。B7-H1表达与胃癌的浸润深度、周围淋巴结转移、远处转移及pTNM分期有关，而PD-1与其无关。结论胃癌组织中B7-H1和PD-1表达升高，B7-H1／PD-1信号通路可能抑制抗肿瘤免疫。B7-H1的表达与胃癌的病程有关，有可能作为判断胃癌预后的指标和胃癌治疗的靶点。Objective The B7-H1/PD-1 co-signaling pathway has recently been found to play a pivotal role in the immune evasion of tumor cells from host immune system. The aim of this study was to examine the B7-H1 and PD-1 expression and TILs status in gastric cancer and to elucidate the clinical relevance of B7-H1 and PD-1 to the pathogenesis of gastric carcinoma. Methods Immunohistochemistry and ANAE histochemical staining were used to investigate the in situ expression of B7-H1 and PD-1 and TILs status in the gastric tissues. RT-PCR was used to explore B7-H1 and PD-1 expression at the transcriptional level. The B7-H1 expression at protein level was detected by Western blot. Results Expression of B7-H1 and PD-1 was found to be increased in gastric carcinoma, but absent in normal gastric tissue. B7-H1 expression in gastric carcinoma was inversely correlated with TILs infiltration. B7-H1 but not PD-1 expression in tumor tissue was significantly correlated with some clinicopathhological variables including depth of invasion, lymph node metastasis and distant metastasis. Conclusion B7-H1 and PD-1 expressions are increased in gastric carcinoma. This signaling pathway may inhibit antitumor immune responses in gastric carcinoma. BT-H1 expression plays a critical role in the pathogenesis of human gastric carcinoma,and might be a promising prognostic marker and therapeutic target in the treatment of this disease.

关 键 词：胃肿瘤 B7-H1 PD-1 淋巴细胞 肿瘤浸润 

分 类 号：R686[医药卫生—骨科学]