中国“人-猪-禽”基因重排H1N2亚型猪流感病毒全基因克隆及遗传进化的研究  

Phylogenetic analysis of human/swine/avian gene reassortant H1N2 influenza A virus isolated from a pig in China

作　　者：陈义祥[1] 蒙雪琼[2] 刘棋[1] 黄夏 黄胜斌[1] 刘翠权[1] 施开创[1] 郭建刚[1] 陈芳芳[1] 胡丽萍[1] 

机构地区：[1]广西动物疫病预防控制中心,南宁530001 [2]广西大学动物科学技术学院,南宁530005 [3]广西动物卫生监督所,南宁530005

出　　处：《微生物学报》2008年第4期466-472,共7页Acta Microbiologica Sinica

摘　　要：【目的】为了研究2006年从广西病猪肺组织中分离的H1N2亚型猪流感病毒(SIV)A/Swine/Guangxi/13/2006(H1N2)(Sw/GX/13/06)的遗传学特性和8个基因的来源。【方法】运用RT-PCR方法对其全基因进行了克隆并运用分子生物学软件对其基因序列进行了遗传进化分析。【结果】血凝素(HA)、核蛋白(NP)、基质蛋白(M)和非结构蛋白(NS)基因来源于猪古典H1N1亚型流感病毒;神经氨酸酶(NA)和聚合酶蛋白(PB1)基因来源于人的H3N2亚型流感病毒;聚合酶蛋白(PA)和聚合酶蛋白(PB2)基因来自于禽流感病毒。【结论】可见Sw/GX/13/06是一株"人-猪-禽"三源基因重排H1N2亚型SIV,且与美国(1999?2001年)和韩国(2002年)分离到该型病毒的有明显的亲缘关系。据我们所知,这是中国首次报道含有禽流感病毒基因片段的重排H1N2SIV,该病毒是否对养猪业和人类公共卫生健康具有潜在的威胁,有待于进一步研究。[Objective] Our aim in this study was to determin the genetic characterization and probable origin of the HIN2 swine influenza virus （A/Swine/Guangxi/13/2006） （Sw/GX/13/06） from lung tissue of a pig in Guangxi province, China. [Methods]Eight genes of Sw/GX/13/06 were cloned and genetically analyzed. [Results] The hemagglutinin （HA）, nucleoprotein （NP）, matrix （M） and non-structural （NS） genes of Sw/GX/13/06 were most closely related to genes from the classical swine H1N1 influenza virus lineage. The neuraminidase （NA） and PB1 genes were most closely related to the corresponding genes from the human influenza H3N2 virus lineage. The remaining two genes PA and PB2 polymerase genes were most closely related to the genes from avian influenza virus lineage. [Conclusion] Phylogenetic analyses revealed that Sw/GX/13/06 was a human/swine/avian H1N2 virus, and closely related to H1N2 viruses isolated from pigs in United States （1999-2001） and Korea （2002）. To our knowledge, Sw/GX/13/06 was the first triple-reassortant H1N2 influenza A virus isolated from a pig in China. Whether the Sw/GX/13/06 has a potential threat to breeding farm and human health remains to be further investigated.

关 键 词：猪流感病毒 H1N2亚型 基因重排 遗传进化 

分 类 号：S852.65[农业科学—基础兽医学]