水母毒素的分离提取及溶血活性研究  

Study on the Extraction and Haemolytic Activity of Jellyfish Venom

作　　者：高健[1] 金义翠[1] 

机构地区：[1]连云港职业技术学院,江苏连云港222006

出　　处：《时珍国医国药》2008年第3期569-570,共2页Lishizhen Medicine and Materia Medica Research

基　　金：江苏省连云港市支柱产业科技攻关项目(No.CN0540);江苏省海洋生物技术重点建设实验室开放基金项目(No.2006HS007)

摘　　要：目的研究水母毒素的提取及其溶血活性的影响因素。方法通过缓冲溶液浸取、超声波细胞破碎等操作从水母触手的刺丝囊中提取毒素,采用分光光度法对水母毒素的溶血活性进行测定,并研究了温度、浓度和二价金属离子等因素对毒素溶血活性的影响。结果水母毒素的溶血活性对温度敏感,随着浓度的提高而增大,并且依赖于Ca2+,Mg2+等二价金属离子的存在。结论水母毒素具有较强的溶血活性,但不稳定。Objective To study the extraction and the affecting factors to haemolytic activity of jellyfish venom, Methods Jellyfish venom was extracted from the oral arms with tentacles through such operations as soaking in buffer solution and disrupting cell in supersonic wave, Activity of the venom to chicken red blood cells was detected by speetrophotometrie method. The effects of factors such as temperature, concentration, divalent metal cations on the haemolytie activity were studied. Results The haemolytie activity was temperature - sensitive, and improved with the concentration increase. The presence of Ca^2＋ , Mg^2＋ wass necessary for haemolytie activity, Conclusion The haemolytie activity of jellyfish venom is relatively strong, but it is unstable.

关 键 词：水母毒素 提取 溶血活性 

分 类 号：R284.2[医药卫生—中药学]