柔肝散结法对肝癌大鼠干预作用的研究  

The Effect of RGSJF on Rats with Liver Cancer

作　　者：范焕芳[1] 雷树恩[2] 黄茂[2] 

机构地区：[1]河北医科大学中医院,河北石家庄050011 [2]河北体育学院,河北石家庄050041

出　　处：《时珍国医国药》2008年第3期588-589,共2页Lishizhen Medicine and Materia Medica Research

基　　金：河北省中医药管理局科研项目(No.2006115)

摘　　要：目的探讨血管内皮生长因子(VEGF)及环氧合酶-2(COX-2)在肝癌大鼠中的表达以及柔肝散结法对其表达的影响。方法40只雄性SD大鼠,随机分为空白组、模型组、青消方组、青消方防治组(简称"防治组"),每组10只。除空白组外,其余3组大鼠均以含0.05%2-AAF的饲料喂养12周,防治组在诱癌的同时灌服青消方。青消方组于诱癌第4周开始给药,剂量均为25 g.kg-1.d-1。12周末观察瘤体积及瘤重量的变化,并采用免疫组织化学方法检测各组大鼠标本中VEGF及COX-2的表达。结果模型组VEGF及COX-2的表达明显高于空白组(P<0.01);青消方组、防治组VEGF及COX-2的表达明显低于模型组(P<0.01);防治组优于青消方组。结论VEGF及COX-2在肝癌大鼠中表达增强,柔肝散结中药青消方能降低肝癌大鼠VEGF及COX-2的表达,早期治疗效果更佳。Objective To approach the expression of the VEGF and COX - 2 in rats with liver cancer and the effect of RouGan-SanJie Fa（RGSJF） on it. Methods 40 SD rats were divided into four groups at random：blank group, model group, QingXiao Fang group, FangZhi group, 10 rats in every group. Except for blank group, the other three groups were fed with bait vessel with 0.05% 2 - AAF for 12 weeks. FanZhi group was lavaged with QingXiao Fang when cancer was induced. QingXiao Fang group 25 g · kg · d^-1 was treated at the 4th week after cancer was induced. The volume and the weight of neoplasm were observed at the 12th week, and the expression of VEGF and COX -2 in every group was detected by immunehistochemical method. Results The expression of VEGF and COX-2 in model group was obviously higher than that of blank group （P 〈0.01 ）. The expression of VEGF and COX -2 in QingXiao Fang group and FangZhi group was obviously lower than that of model group （P 〈 0.01 ）, FangZhi group surpassed QingXiao Fang group. Conclusion The expression of VEGF and COX -2 was augmented in rats with liver cancer, QingXiao Fang can depress the expression of VEGF and COX -2 in rats with liver cancer, especially at earlier period.

关 键 词：柔肝散结法 肝癌大鼠 血管内皮生长因子 环氧合酶-2 

分 类 号：R285.5[医药卫生—中药学]