家兔血浆中北豆根碱的高效液相色谱法测定及药动学研究  

Study on HPLC Determination Method and Pharmacokinetics of Dauricine in Rabbits

作　　者：朱孝芹[1] 臧亚如[1] 张欢 刘翠哲[3] 

机构地区：[1]承德医学院附属医院,河北承德067000 [2]河北省承德市疾病控制中心,067000 [3]承德医学院中药研究所,067000

出　　处：《时珍国医国药》2008年第3期596-597,共2页Lishizhen Medicine and Materia Medica Research

基　　金：河北省卫生厅医药开发重大资助项目(No.04006)

摘　　要：目的建立家兔血浆中北豆根碱（RM）的HPLC分析方法，并对RM静注给药在家兔体内药动学过程进行分析研究。方法采用乙腈、二氯甲烷沉淀蛋白质并提取药物，采用HPLC法测定。色谱柱为DiscoveryC18柱（4．6mm×250mm，5μm）；流动相为乙腈：水：磷酸=16：84：0．15（V：V）；检测波长208nm；柱温为室温；流速0．5ml·min^-1；动物实验使用家兔6只，采用静脉注射给药（10mg·kg^-1），进行血药浓度测定和药动学参数计算。结果家免血浆内源物质对药物测定无干扰，在血药浓度0．01-0．10mg·ml^-1范围内，浓度对峰面积有良好的线性关系，r=0．9998，提取回收率大于85％，方法回收率在98．3％～100．5％之间；日内、日间精密度RSD均小于10％（n=5）。北豆根碱在家兔体内的血药浓度-时间曲线符合二室模型。结论该法准确、简便、灵敏，可用于北豆根碱在兔体内药动学的研究。Objective To establish an HPLC method for determination of dauricine in rabbits plasma and study the related pharmacokinetics in rabbits after IV injection. Methods After protein was precipitated with acetonitrile and dichloromethane, dauricine in plasma was determined on HPLC with UV detection at 208 nm, and the mobile phase was acetonitrile - water - phosphoric acid （ 16： 84： 0.15）, running at a flow rate of 0.5 ml · min^-1. We detected the concentration of dauricine in plasma and caculated pharmacokinetic parameters by 6 rabbits after IV injection （ 10 mg · kg^-1 ）. Results Dauricine and endogenous could be separated. Its calibration curve was linear（ r = 0.999 8） within range of 0.01 -0.1 mg· ml^-1, the extraction recovery was more than 85% , the method recovery was within range of 98.3% - 100.5% , the intra - day and inter - day precision was less than 10% （ n = 5 ）. The concentration - time curve of dauricine in rabbits plasma could be fitted to two - compartment model. Conclusion This method is sensitive, simple and accurate. It is suitable for the pharmacokinetic study of dauricine in rabbits.

关 键 词：北豆根总碱 高效液相色谱法 家兔 药动学 

分 类 号：R962[医药卫生—药理学]