槐米固态发酵提高槲皮素含量的研究  

Study on Increasing the Content of Quercetin in Flos Sophorae Immaturus by Solid State Fermentation

作　　者：徐萌萌[1] 沈竞[1] 徐春[1] 王东溯[1] 汪红[1] 孙启玲[1] 

机构地区：[1]四川大学生命科学学院.生物资源与生态环境教育部重点实验室,四川成都610064

出　　处：《时珍国医国药》2008年第3期704-706,共3页Lishizhen Medicine and Materia Medica Research

摘　　要：目的用该实验室筛选得到的一株黑曲霉固态发酵槐米并进行了培养条件优化,使其将槐米中的芦丁转化为槲皮素,提高槐米中槲皮素的含量。方法使用正交实验对种子培养条件及发酵条件进行优化。结果经优化后的菌体量可达8.32 g/L,优化的固态发酵条件芦丁转化率可达到98%以上。利用高效液相色谱法对发酵产物进行了鉴定,证明转化产物为槲皮素。结论通过微生物固态发酵槐米的方法,能够提高槐米中槲皮素的含量。Objective Flos Sophorae Immaturus was subjected to solid state fermentation process by an Aspergillus niger strain to transform rutin into quercetin. Methods The conditions of culture and fermentation process were optimized by the orthogonal layout. Results By modulating the conditions, the biomass could reach 8.32 g/L and the transformation rate of rutin could reach 98%. It was proved that the product was quercetin by HPLC. Conclusion The content of quercetin in Flos Sophorae Immaturus can be increased by the method of microbial transformation.

关 键 词：槐米 芦丁 槲皮素 黑曲霉 微生物转化 

分 类 号：R284.1[医药卫生—中药学]