草木樨属根瘤菌的16S rDNA-RFLP分析  

Analysis of Rhizobia Isolated from Melilotus by 16S rDNA-RFLP

作　　者：李香香[1] 张美玲[1] 付芸芸[1] 赵美玲[1] 韦革宏[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西省农业分子生物学重点实验室,陕西杨陵712100

出　　处：《西北植物学报》2008年第3期569-573,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30670372);全国优秀博士学位论文作者基金(200254);教育部新世纪优秀人才支持计划基金(NCET-04-0953);西北农林科技大学拔尖人才支持计划基金

摘　　要：采用16S rDNA-RFLP分析方法对草木樨属根瘤菌进行了遗传多样性及系统分类研究。结果表明,3种限制性内切酶(HaeⅢ、HinfⅠ、MspⅠ)对所有供试菌株的酶切图谱类型组合只有2种。类型I的代表菌株CC-NWSX0003-1与豌豆根瘤菌(R.leguminosarum)的模式菌株USDA2370的序列相似性达到99.8%,在分类地位上属于根瘤菌属(Rhizobium)分支;类型Ⅱ的代表菌株CCNWGS0006与草木樨中华根瘤菌(Sinorhizobium meliloti)的16S rDNA相似性为100%,属于中华根瘤菌属(Sinorhizobium)分支。The genetic diversity and taxonomy of the rhizobia isolated from nodules of Melilotus were studied by 16S rDNA-RFLP. The result showed that there were only two types of fingerprint patterns among all tested strains by three restriction endonucleases （Hae Ⅲ, Hinf Ⅰ, Msp Ⅰ ）. The representative strain CCNWSX0003-1 of type Ⅰ, which fell into Rhizobium, shared 99.8% sequence homology with R. leguminosarum USDA2370. The strain CCNWGS0006 of type Ⅱ was highly related to Sinorhizobium meliloti at the similarity level of 100% and belonged to the genus Sinorhizobium.

关 键 词：根瘤菌 草木樨属 RFLP 系统分类 

分 类 号：Q939.114[生物学—微生物学]