二阶校正法与三维荧光光谱相结合直接测定人体血浆中的水杨酸  

Determination of salicylate acid in plasma using three-dimensional fluorescence coupled with second-order calibration algorithms

作　　者：边英超[1] 吴海龙[1] 夏阿林[1] 朱绍华[1] 俞汝勤[1] 

机构地区：[1]湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,湖南长沙410082

出　　处：《计算机与应用化学》2008年第3期257-260,共4页Computers and Applied Chemistry

基　　金：国家自然科学基金(20475014，20775025；20435010)

摘　　要：水杨酸、萘普生及血浆背景干扰组分,这三者荧光光谱重叠严重,如果不经分离,无法用常规方法定量分析水杨酸。本文利用二阶校正的优势,将三维激发发射荧光光谱与平行因子分析算法和交替三线性分解算法相结合,快速分析测定血浆中的水杨酸。当因子数选为4时,用两种算法获得的回收率分别为(103.8±3.5)%和(98.4±1.4)%。实验结果表明,此方法可用于干扰组分共存下,快速定量测定血浆中的水杨酸,从而可用于研究阿斯匹林的代谢过程。In this paper,the methodologies of combining excitation-emission fluorescent spectra with second-order calibration based on both the alternating trilinear decomposition（ATLD）algorithms and the parallel factor analysis（PARAFAC）algorithms were applied to directly determine salicy late acid（SA）in plasma even in the presence of other uncalibrated interferents.When the number of the com- ponents was set to 4,the obtained recoveries were（103.8±3.5）% for PARAFAC and（98.4±1.4）% for ATLD,respectively.The satisfactory results indicated that the proposed methods could he applied to simply quantifying SA in human plasma even in the presence of unknown interferences.

关 键 词：水杨酸 血浆 萘普生 激发发射矩阵荧光法 二阶校正 交替三线性分解算法 平行因子分析算法 

分 类 号：O656[理学—分析化学]