铅污染对牙鲆GSH-PX酶活性的影响  

Effect of Pb on activities of GSH-PX enzyme in tissues of Paralichthys olivaceus

作　　者：王凡[1] 赵元凤[2] 吕景才[2] 刘长发[2] 

机构地区：[1]洛阳师范学院生命科学系,471022 [2]农业部海洋水产增养殖学与生物技术重点开放实验室

出　　处：《海洋水产研究》2008年第1期20-23,共4页Marine Fisheries Research

基　　金：国家自然科学基金项目(30271029)资助

摘　　要：将牙鲆置于浓度为0.5mg/L的Pb溶液中18d,测定其鳃、肌肉、全部内脏中谷胱甘肽过氧化氢酶(GSH-PX)的活性。结果表明,最初8d,各组织的GSH-PX的酶活性表现为抑制,随后各组织GSH-PX的酶活性表现为诱导;10d时,诱导幅度最大,而后表现为抑制。紧接着放入清洁海水,各组织的酶活性又恢复到对照组的水平。结果提示,对于低浓度铅的长期暴露,GSH-PX能明显指示牙鲆受海洋铅的早期污染。The effect of long term exposure to Pb （0.5mg/L） on activities of protecting enzyme GSH-PX in tissues （gill, muscle and visceral organs） of Paralichthys olivaceus was studied. It was shown that the activities of protecting enzyme GSH-PX in all tissues of P. olivaceus was inhibited on the 8th day, and was induced on the following days; on the 10th day, enzyme activity induction was at the highest level, and was inhibited again thereafter. Then after a few days＇ cultivation in clean water, activity of protecting enzyme GSH-PX in all tissues was reduced to the initial level. These results also suggested that GSH-PX could be used as a bio-indicator of low concentration Pb^2＋ exposure in aquatic organisms.

关 键 词：铅污染 牙鲆 谷胱甘肽过氧化物酶 

分 类 号：S948[农业科学—水产养殖] X52[农业科学—水产科学]