低温及胰酶加Ca^(2+)对中华绒螯蟹精子顶体反应的诱导  

Induction of acrosome reaction by low temperature and trypsin-Ca^(2+) on spermatozoa of Eriocheir sinensis

作　　者：李根亮[1] 康现江[1] 曹刚[1] 穆淑梅[1] 葛少钦[1] 

机构地区：[1]河北大学生命科学学院,保定071002

出　　处：《海洋水产研究》2008年第1期24-28,共5页Marine Fisheries Research

基　　金：国家自然科学基金项目(30371115);河北省自然科学基金项目(303118)共同资助

摘　　要：研究了不同温度及不同Ca2+浓度提取缓冲液加0.1%胰酶(w/v)对中华绒螯蟹Eriocheirsinensis精子顶体反应发生的诱导效果。结果表明,-20、-80和-196℃处理精子不同时间和加0.1%胰酶的含Ca2+的人工海水作精子提取缓冲液提取精子均可有效诱导精子发生顶体反应;甚至在不含Ca2+的精子悬液中,冷冻精子也能大量发生顶体反应;而未处理的对照组和4℃处理的精子无此现象,不同的Ca2+浓度梯度对精子顶体反应率的影响没有明显的差异性;不含Ca2+的人工海水中的胰酶不能诱导顶体反应。Effects of different temperatures and Ca^2＋ concentration butters added with 0. 1% trypsin（w/v）on acrosome reaction （AR） induction were investigated for spermatozoa of Chinese wooly-handed crab Eriocheir sinensis. It was found that AR was induced by - 20, - 80 and --96 ℃ preservation for different time periods, as well as by artificial seawater containing Ca^2＋ and 0. 1% trypsin. AR also happened profusely in spermatozoa suspensions without Ca^2＋ , while the control and 4℃ treatment did not induce AR. No significant difference was found for AR induction by different Ca^2＋ concentrations, and trypsin in artificial seawater without Ca^2＋ did not induce AR.

关 键 词：中华绒螯蟹 精子 顶体反应 诱导 

分 类 号：Q492[生物学—生理学] S917[农业科学—水产科学]