利用DD-PCR技术分析水稻铝诱导基因的表达差异  

The Expressive Difference of Inductive Gene by Aluminium in Rice by Differential Display

作　　者：张立平[1] 吴平[1] 祝金明[1] 罗安程[1] 

机构地区：[1]浙江农业大学生物科技系

出　　处：《中国农业科学》1997年第5期71-74,共4页Scientia Agricultura Sinica

基　　金：国家教委优秀青年教师基金

摘　　要：利用差异显示（ＤｉｆｅｒｅｎｔｉａｌＤｉｓｐｌａｙＰＣＲ，简称为ＤＤ－ＰＣＲ）技术比较了水稻对铝极敏感的品种ｐｐ２４６２－１１和极抗品种Ｐｅｄｅｌ在铝胁迫条件下基因的表达差异。结果表明：抗性品种和敏感品种在铝胁迫下其苗期ｍＲＮＡ存在明显差异，实验共发现２５个差别ｃＤＮＡ，铝既可诱导抗性品种和敏感品种的基因表达，又可抑制其基因表达。本研究推测抗性品种特异表达的片段可能与合成抗性蛋白有关，敏感品种基因的特异表达可能产生一些毒害蛋白，抑制根的生长。The expressive difference of gene under aluminum stress was analysed in sensitive genotype pp2462 11 and resistant cultivar Pedel by Differential Display PCR. The results indicated great difference in cDNA of expressed gene between the resistant and sensitive rice to aluminum. 25 special cDNA fragments were found. It is suggested that the special cDNA fragments are related to resistiant protein in resistant genotype, and the special cDNA in sensitive cultivar may produce toxic protein, which inhibits the growth of root.

关 键 词：水稻 基因表达 差异显示 铝诱导 

分 类 号：S511.035.2[农业科学—作物学]