青龙衣多糖对S_(180)小鼠红细胞内阳离子的影响  

Effect of Qinglongyi Polysaccharide on Cation Concentration in Erythrocyte of S_(180) Mice

作　　者：季宇彬[1] 肖凤[2] 汲晨锋[1] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研发中心,黑龙江哈尔滨150076 [2]哈尔滨商业大学药物研究所博士后科研工作站,黑龙江哈尔滨150076

出　　处：《药品评价》2008年第2期86-88,共3页Drug Evaluation

基　　金：国家自然科学基金项目(30600816);高等学校博士学科点专项科研基金(20060240001)

摘　　要：目的考察青龙衣多糖对S_(180)小鼠红细胞内Ca^(2+)和H^(+)的影响。方法建立肿瘤动物模型,分高、中、低剂量腹腔给予青龙衣多糖7d,采集红细胞,制备红细胞悬液。应用激光扫描共聚焦显微镜结合荧光探针Fluo-3/AM染色观察和测定S_(180)小鼠红细胞内Ca^(2+)的变化,结合荧光探针BCECF-AM染色观察和测定S_(180)小鼠红细胞内H+的变化。结果青龙衣多糖能降低S_(180)小鼠红细胞内Ca^(2+),与阴性对照组相比较有显著性差异(P<0.05);青龙衣多糖各剂量组均能显著降低S_(180)小鼠红细胞膜内的H^+浓度,与阴性对照组相比较有显著性差异(P<0.01)。结论青龙衣多糖组能维持S_(180)小鼠红细胞内阳离子浓度稳定平衡。Objective To study the effect of qinglongyi polysaccharide on cation concentration in erythrocyte of S180 mice. Methods A tumor mouse model was developed, and the mice were treated with high, medium, and low doses of qinglongyi polysaccharide through abdominal injection for 7 d. Erythrocytes were collected and erythrocyte suspensions were prepared. Change of Ca^2＋ concentration in the erythrocytes ofS180 mice was measured by stained with Fluo-3/AM and observed with laser scanning confocal microscope, Change of H＋ concentrations measured by stained with BCECF-AM and observed with laser scanning confocal microscope. Results Qinglongyi polysaccharide lowered the concentration of Ca2＋ and H＋ in erythrocytes of S180 mice, which is remarkable compared with negative control group（P〈0.05）. Conclusion Qinglongyi polysaccharide can maintain the stability and balance of cation concentration in erythrocyte of S180 mice.

关 键 词：青龙衣多糖 红细胞 阳离子 小鼠 

分 类 号：R285[医药卫生—中药学]