TCDD对斑马鱼脂质过氧化作用的初步研究  

Studies on Lipid Peroxidation of TCDD in Zebrafish

作　　者：刘连平[1] 聂芳红[2] 孔庆波[3] 林红英[1] 谢英明[1] 杨蓉[1] 陈进军[1] 

机构地区：[1]广东海洋大学农业生物技术研究所,广东湛江524088 [2]广东海洋大学食品科技学院,广东湛江524088 [3]中国刑事警察学院警犬技术系,辽宁沈阳110034

出　　处：《广东海洋大学学报》2008年第1期81-84,共4页Journal of Guangdong Ocean University

基　　金：广东省自然科学基金项目"几种DLCs对斑马鱼CYP1A的生化毒理学机理研究"(05011789);广东省国际合作项目"斑马鱼生物学标志CYP1A在DLCs环境污染监测中的应用研究"(2007B050200023);广东海洋大学SCI刊源作者基金项目

摘　　要：将150条斑马鱼随机等分为5组:2,3,7,8-四氯代二苯并二噁英(TCDD)4个染毒组(染毒剂量分别为0.1μg/L、0.2μg/L、0.4μg/L和0.8μg/L)和空白对照组,水质接触染毒5d后,采用分光光度法测定其肝组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)、谷胱甘肽-S-转移酶(GST)的活力,研究TCDD对斑马鱼的脂质过氧化作用。结果发现,TCDD各剂量染毒组MDA含量均有所增加,其中0.2μg/L组差异显著(P<0.05),0.4μg/L和0.8μg/L剂量组差异极显著(P<0.01);各染毒组SOD活性均有所下降,其中0.2μg/L、0.4μg/L和0.8μg/L剂量组差异极显著(P<0.01);各染毒组GST活力均降低且差异极显著(P<0.01)。一定剂量的TCDD能引起MDA含量增加,使SOD和GST活力降低,对斑马鱼具有脂质过氧化作用。To study the lipid peroxidation of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin （TCDD） in zebrafish, 150 zebrafish were randomly and equally divided into 5 groups： four test groups were exposed to TCDD at 0.1 μg/L, 0.2 μg/L, 0.4 μg/L, 0.8 μg/L , respectively in addition to one control group. Five days after the animals were exposed to the polluted water with different doses of TCDD, respectively, the level of malondialdehyde （MDA） and the activities of superoxide dismutase （SOD） and glutathionetransferase （GST） in liver of zebrafish were determined by means of spectrophotometry. It was found that the MDA level showed some increasing tendency in which 0.2 μg/L of TCDD exposure group was different （P〈0.05） and 0.4μg/L and 0.8 μg/L of TCDD exposure groups were significantly different from the control group （P〈0.01）, that SOD activity showed decreasing tendency and 0.2 μg/L, 0.4 μg/L and 0.8 μg/L of TCDD exposure groups were significantly different from control group （P〈0.01）, and that GST activity in all TCDD treated groups showed significantly difference from the control group （P〈0.01）. It wasconcluded that TCDD at certain doses caused hepatic lipid peroxidation in zebrafish by induction of MDA increase and SOD and GST inactivation.

关 键 词：斑马鱼 丙二醛 超氧化物歧化酶 谷胱甘肽-S-转移酶 2 3 7 8-四氯代二苯并二噁英 

分 类 号：X503.225[环境科学与工程—环境工程]