抑颤汤对帕金森病大鼠脑内多巴胺受体和神经降压素含量的影响  

Effects of Yichan decoction(抑颤汤) on dopamine receptors and neurotensin in cerebral tissue of rats with Parkinson's disease

作　　者：杨明会[1] 王海明[1] 刘毅[1] 窦永起[1] 

机构地区：[1]解放军总医院中医研究所,北京100853

出　　处：《中国中西医结合急救杂志》2008年第2期71-73,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金资助项目(30672762)

摘　　要：目的探讨抑颤汤治疗帕金森病(PD)的作用机制。方法用直接向脑组织内注入神经毒剂6-羟基多巴损毁脑黑质致密部的方法建立PD大鼠模型。将100只SD大鼠随机分为正常对照组、PD模型组和抑颤汤治疗组,连续治疗8周。观察各组治疗前及治疗5周起PD大鼠旋转行为特征的变化;用放射免疫法测定治疗后大鼠脑组织多巴胺(DA)受体亲和常数(Kd)和最大结合量(Bmax)以及神经降压素免疫活性物(NT-IR)含量的变化。结果抑颤汤能明显改善PD模型大鼠的旋转行为,使治疗后大鼠每40 min的平均旋转次数从(349.56±62.28)圈降为(61.63±17.93)圈,P<0.01〕。与模型组比较,抑颤汤组大鼠损毁侧脑组织DA受体Bmax和Kd明显提高〔Bmax:(304.350±19.328)nmol/kg比(264.630±22.124)nmol/kg,P<0.05;Kd:(1.476±0.546)nmol/L比(1.248±0.658)nmol/L,P<0.01〕;损毁侧脑组织NT-IR含量较模型组显著降低,差异有统计学意义〔(72.37±29.15)μg/kg比(152.68±26.21)μg/kg,P<0.01〕。结论抑颤汤能调节PD模型大鼠大脑释放神经降压素,增加脑内DA含量,促进受损脑黑质神经细胞修复,从而改善PD大鼠的旋转行为。Objective To explore the mechanism of Yichan decoction （抑颤汤） in treatment of Parkinson＇s disease （PD）. Methods A PD rat model was made by direct injection of 6-hydroxydopamine （6-OHDA） into the brain to destruct the compact part of substantia nigra （SNC）. One hundred Sprague- Dawley （SD） rats were randomly divided into a normal control group, a PD model group and a Yichan decoction treatment group and they were treated for 8 weeks. The rats＇ behavior pattern changes were observed before treatment and from 5th weeks after treatment. The changes in the dissociation constant （Kd） and maximum number of binding sites （Bmax） of dopamine （DA） receptors, and neurotensin-immunoreactive （NT-IR） in substantia nigra after treatment were determined by using the technique of radioimmunity. Results Yichan decoction could markedly improve the rotation of PD model rats and decrease the average number of rotation from （349.56± 62.28） circle/40 minutes to （61.63± 17.93） circle/40 minutes （P〈0. 01）. The Bmax and Kd of DA receptors in the brain tissues on the lesion side of Yichan decoction group were more improved obviously than those of the PD model group （Bmax： （304. 350± 19. 328） nmol/kg vs. （264. 630±22. 124） nmol/kg, P〈0. 05; Kd： （1. 476± 0. 546） nmol/L vs. （1. 248± 0. 658） nmol/L, P〈0. 013. The content of NT-IR of the rats＇ lesion side was significantly less in Yichan decoction group than that in the PD model group [（72.37±29.15） μg/kg vs. （152.68±26.21） μg/kg, P〈0. 01]. Conclusion Yichan decoction can regulate the release of neurotensin from the cerebrum, increase the DA content in the brain, and promote the repair of the injured black substance neural cells in the PD model rat, thus the behavior of PD rat rotation is improved.

关 键 词：帕金森病 作用机制 抑颤汤 

分 类 号：R285.5[医药卫生—中药学] R742.5[医药卫生—中医学]