孕妇外周血中单个胎儿有核红细胞产前诊断胎儿ABO血型  

Fetal ABO genotyping using single NRBCs in maternal peripheral blood

作　　者：付先虎[1] 陈汉平[1] 阎小芳[1] 余蓉[1] 刘燕燕[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,湖北武汉430030

出　　处：《中国妇幼保健》2008年第9期1217-1219,共3页Maternal and Child Health Care of China

摘　　要：目的:探讨母血中单个胎儿有核红细胞(NRBCS)产前诊断胎儿ABO血型的可行性。方法:从154例孕妇外周血中分离单个NRBCS,应用引物延伸扩增(PEP)法扩增单个NRBCS的基因组,用PCR法检测扩增产物中的SRY基因以判断NBRCS的来源。再用特异性序列-聚合酶链反应(PCR-SSP)法测胎儿ABO血型基因型。抽取新生儿脐血提取淋巴细胞,应用PCR-SSP法测胎儿ABO血型基因型。用常规血清方法测新生儿血型。结果:用PCR-SSP法检测出154例中的152例血型,2例未测出,检出率98.7%。检测出的152例胎儿基因型与脐血淋巴细胞所测ABO血型基因型完全相符。常规血清学方法测新生儿ABO血型,检测率为85.7%。结论:用单个NRBCS结合PCR-SSP法检测胎儿ABO血型方法可靠。Objective： To assess the feasibility of fetal ABO genotyping with NRBCs in maternal peripheral blood. Methods： Mi- eromanipulation techniques were employed to isolate NRBCs from 154 maternal blood samples. NRBCs were tested for SRY gene and ABO gene by PEP and PCR -SSP. Routine serological method was used to test ABO phenotype of mothers and newborns. Lymphoeytes isolated from umbilical cord blood were tested for ABO genotyping of newborns. Results： NRBCs were found in all samples. The detection rate of ABO genotyping with NRBCs was 98. 7% while detection rate of newborn blood group by serological method was 85. 7%. Conclusion： The detection rate of fetal genotyping with NRBCs is higher than that with serological method, and it is feasible to diagnose fetal ABO genotype.

关 键 词：胎儿血型NRBCS PCR-SSP无创性产前诊断 

分 类 号：R714[医药卫生—妇产科学]