山茶属油茶组、短柱茶组和红山茶组植物叶的 红外光谱分析及其分类学意义(英文)  

FTIR spectra of Camellia sect. Oleifera, sect. Paracamellia, and sect. Camellia (Theaceae) with reference to their taxonomic significance

作　　者：沈锦波[1] 吕洪飞[1] 彭秋发[1] 郑菊芳[2] 田玉梅[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,金华321004 [2]浙江师范大学物理化学研究所浙江省固体表面反应化学重点实验室,金华321004

出　　处：《植物分类学报》2008年第2期194-204,共11页Acta Phytotaxonomica Sinica

摘　　要：利用傅立叶红外光谱仪和OMNI采样器直接、迅速、准确地测定山茶属Camellia4组63种2变种植物叶片的红外光谱,结果表明:各分类群(种)的红外光谱具有高度特异性,其红外光谱图的变化可以作为山茶属植物属下的分类依据之一。这也暗示了利用标准红外光谱图库,可以区分和鉴定出山茶属植物的种类。经主成分分析后的红外光谱数据构建的树型聚类图与先前的形态分类结果大体一致,能将油茶组sect.Oleifera和短柱茶组sect.Paracamellia植物明显区分,并且各组中亲缘关系较近的种聚在一起。因此支持它们作为两个独立的组处理。但是,红山茶组sect.Camellia内的滇山茶亚组subsect.Lucidissima和光果红山茶亚组subsect.Reticulata植物在聚类图上很难区分,建议将这两个亚组植物进行归并。最后讨论了张宏达和闵天禄系统中存在分歧的油茶组、短柱茶组和红山茶组内的种间分类关系。Sixty-five leaf samples in sect. Oleifera H. T. Chang, sect. Paracamellia Scaly, sect. Camellia, and sect. Thea （L.） Dyer of genus Camellia L. were discriminated directly with an OMNI-sampler accessory on the basis of biochemical profiles and a hierarchical dendrogram was finally constructed. The results showed that the infrared spectra of Camellia were fingerprint-like patterns which were highly typical for different taxa. The hierarchical dendrogram based on principal component analysis of Fourier Transform Infrared （FTIR） data confirmed most of morphological classifications of the four sections proposed in previous works. Infrared spectra of leaves are of taxonomic value in genus Camellia, and this technique can be widely used for identification and classification of other taxa when standard spectra are available. The relationships between sect. Oleifera and sect. Paracamellia, subsect. Lucidissima H. T. Chang and subsect. Reticulata H. T. Chang in sect. Camellia, and the species/varieties were also discussed, as many dissensions about the classification exist between Chang＇s and Ming＇s system.

关 键 词：山茶属 聚类分析 红外光谱 分类学意义 

分 类 号：Q946[生物学—植物学] Q949