胆汁酸合成增加与肝D-双功能蛋白表达增强、活性升高的相关性  

Correlation of bile acid synthesis increase with hepatic DBP mRNA level and activity

作　　者：王素玲[1] 王切[2] 姜玲玲[1] 

机构地区：[1]河北医科大学基础医学院生化教研室,河北石家庄050017 [2]河北医科大学基础医学院解剖教研室,河北石家庄050017

出　　处：《第四军医大学学报》2008年第6期516-518,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金(30371566);河北省自然科学基金(303472)

摘　　要：目的:观察胆汁酸合成增多时,DBP的表达和活性的改变,从整体水平上探讨DBP在胆汁酸合成中的作用.方法:给C57BL/6J小鼠T0901317及给Wistar大鼠饲喂高胆固醇食物,两条途径激活肝X受体(LXR),上调CYP7A1的表达增加胆汁酸的合成后,用全自动生化分析仪测定粪胆汁酸,用RT-PCR的方法测定LXR的下游基因胆汁酸合成限速酶CYP7A1,以及DBP的mRNA水平;用Western Blot测定DBP的蛋白水平;用分光光度法测定DBP活性.结果:LXR被T0901317或高胆固醇食物激活后,CYP7A1的mRNA增加(P<0.05),导致粪中胆汁酸排出增加(P<0.05)的同时,小鼠、大鼠肝DBP的mRNA水平、催化活性以及小鼠的DBP蛋白水平也随之增加(P均小于0.05).结论:DBP在整体水平参与了胆汁酸合成并发挥重要作用.AIM ： To investigate the physiological role of DBP in bile acid biosynthesis through determining the change of its expression and activity when bile acid biosynthesis increases. METHODS： To activate LXR and increase bile acid synthesis, C57BL/6J mice were fed T0901317 for 7 d and Wistar rats were fed a high-cholesterol diet for 2 wk. Fecal bile acid was measured by auto-biochemical-analysis apparatus. The expression of CYPTA1 and DBP mRNA was analyzed by RT-PCR. DBP protein was estimated by Western Blot. DBP activity was measured by spectrophotometry. RESULTS ： Fecal bile acids （ P 〈 0. 05 ） , the mRNA expression of CYPTA1 （P 〈 0. 05 ） and DBP （ P 〈 0.01 ）, and activity of DBP（ P 〈 0.05 ） in liver of C57BL/6J mice treated with T0901317 and Wistar rats fed high-cholesterol diet were higher than that in the corresponding control groups. The liver protein level of DBP in mice treated with T0901317 also increased （ P 〈 0.05 ）. CONCLUSION： DBP is involved in the bile acid biosynthesis in vivo.

关 键 词：D-双功能蛋白 胆汁酸类和盐类/生物合成 肝X受体 胆固醇7Α-羟化酶 

分 类 号：Q591[生物学—生物化学] Q55