血管内皮生长因子在不同乳腺组织中的表达及其意义  

Expression of vascular endothelial growth factor in different breast tissues and clinical significance thereof

作　　者：徐卫国[1] 王刚[2] 刘阳[3] 邹玉环[2] 宋纪宁[1] 杨小青[1] 王文雅[4] 

机构地区：[1]华北煤炭医学院附属医院肿瘤外科,唐山063000 [2]附属开滦医院肿瘤科 [3]唐山工人医院ICU [4]华北煤炭医学院病理系

出　　处：《中华医学杂志》2008年第12期802-804,共3页National Medical Journal of China

基　　金：河北省科学技术研究与发展计划基金资助项目（0527611016）

摘　　要：目的观察乳腺良性病变、不典型增生及原位癌中血管内皮生长因子（VEGF）的表达水平及肿瘤微血管密度（MVD）值的变化与临床病理学特征之间的相关性。方法采用免疫组化染色法（SP法）分别检测100例乳腺良性病变（包括乳腺纤维瘤35例、乳腺囊性增生35例和乳腺导管内乳头状瘤30例）、15例乳腺不典型增生和25例乳腺原位癌患者术后标本中VEGF的表达水平及MVD值。结果乳腺良性病变、乳腺不典型增生和乳腺原位癌标本中VEGF阳性率分别为22％、33％和56％，乳腺良性病变组最低，原位癌组最高，3组VEGF表达水平呈依次升高趋势（P〈0．05）；3组中MVD值分别为14．3±3．5、18．5±3．6和20．1±6．1，良性病变组MVD值最低，原位癌组MVD值最高，3组MVD值呈依次升高趋势（P〈0．05）；在VEGF阳性组中MVD值高于VEGF阴性组（P〈0．05）。结论VEGF在乳腺肿瘤血管生成过程中可能发挥重要的调控作用。VEGF可能与乳腺癌的发生发展有关。Objective To investigate the differences in the expression of vascular endothelial growth factor （VEGF） and mcrovessel density （MVD） count in breast benign affection, breast atypical hyperplasia, and breast carcinoma in situ and to clarify the association of VEGF expression and MVD with the clinicopathological features of these diseases. Methods Immunohistochemistry （SP-method） was used to examine the expression of VEGF and MVD count in 100 samples of breast benign affection （ including 35 cases of breast fibroid tumor,35 cases of breast cystic hyperplasia, and 30 cases of intraduetal papilloma）, and 15 samples of breast atypical hyperplasia, and 25 samples of breast carcinoma in situ, obtained during operation. Results The positive rate of VEGF of the breast carcinoma in situ group was 56%, significantly higher than hose of the breast benign affection and breast atypical hyperplasia groups （22% and 33% respectively, P 〈 0.05）. However, there was no significant differences in positive rate of VEGF among breast fibroid tumor, breast cystic hyperplasia, and intraductal papilloma （all P 〉 0.05 ）. The MVD value of the breast carcinoma in situ group was 20. 1 ± 6. 1, significantly higher than those of the breast benign affection group and breast atypical hyperplasia groups （ 14.3 ± 3.5 and 18.5 ± 3.6 respectively, both P 〈 0.05）. There was no significant differences in MVD value among breast fibroid tumor, breast cystic hyperplasia, and intraductal papilloma （ all P 〉 0.05 ）. Conclusion In breast tumors, angiogenesis is probably mediated mainly by VEGF. The occurrence and progression of breast cancer may be related with the expression of VEGF.

关 键 词：乳腺肿瘤 血管内皮生长因子类 乳腺良性病变 乳腺不典型增生 MVD 

分 类 号：R686[医药卫生—骨科学]