油菜素内酯不敏感型的拟南芥突变型筛选  

Selection of Brassinolide-insensitive Mutant of Arabidopsis thaliana

作　　者：郑慧琼[1] 陈季楚[1] 赵毓橘[1] 许智宏[1] 

机构地区：[1]中国科学院上海植物生理研究所,上海200032

出　　处：《植物生理学报（0257-4829)》1997年第3期293-298,共6页Acta Phytophysiologica Sinica

基　　金：国家自然科学基金!39670077

摘　　要：Arabidopsis thaliana mutants insensitive to brassinolide (BR) were isolatedby screening for growth of seedling ona medium containing epi-BR 0. 05 mg/L. The mutant phenotypically changedin the morphology of roots and leaves(Fig. 2 ). Genetic analysis of thesemutants indicated that the BR insensitivity was due to a recessive mutation,which was designated as br. In addition,studies on the effects of epi-BR onthe growth of leaves of mutunt progenies and pH change of the bathingmedium by using leaf discs showed thatleaves of the mutunt were as insensitiveto epi-BR as roots.Arabidopsis thaliana mutants insensitive to brassinolide (BR) were isolatedby screening for growth of seedling ona medium containing epi-BR 0. 05 mg/L. The mutant phenotypically changedin the morphology of roots and leaves(Fig. 2 ). Genetic analysis of thesemutants indicated that the BR insensitivity was due to a recessive mutation,which was designated as br. In addition,studies on the effects of epi-BR onthe growth of leaves of mutunt progenies and pH change of the bathingmedium by using leaf discs showed thatleaves of the mutunt were as insensitiveto epi-BR as roots.

关 键 词：拟南芥 油菜素内酯 不敏感型突变型 筛选 

分 类 号：Q949.748.3[生物学—植物学]