反相高效液相色谱法测定蟾酥中的3种蟾毒内酯  

Determination of three bufogenins in toad venom using reversed-phase high performance liquid chromatography

作　　者：刘吉华[1] 王静蓉[1] 余伯阳[1] 

机构地区：[1]中国药科大学中药学院中药复方研究室,江苏南京210009

出　　处：《色谱》2008年第2期186-188,共3页Chinese Journal of Chromatography

基　　金：国家自然科学基金资助项目(30572320)

摘　　要：建立了采用反相高效液相色谱同时测定药材蟾酥中华蟾酥毒基、酯蟾毒配基和蟾毒灵含量的方法。采用反相C18色谱柱,以乙腈-0.1mol/L醋酸缓冲液（pH3.2）（体积比为50∶50）为流动相,流速0.8mL/min,在299nm波长下检测。华蟾酥毒基、酯蟾毒配基和蟾毒灵的线性范围分别为0.25-0.875μg（r=0.9990）、0.25-0.875μg（r=0.9991）和0.15-0.525μg（r=0.9990）;平均加样回收率分别为100.3%、100.0%和98.0%,结果表明所建方法简便可靠。对不同来源的10批样品中的3种蟾毒内酯含量的检测结果显示,不同来源或批次的蟾酥药材中3种蟾毒内酯总含量差异较大。A reversed-phase high performance liquid chromatographic method for the quantitative determination of three bufogenins in toad venom was established.An Alltech Alltima C18 column（250 mm×4.6 mm,5 μm）was used.The mobile phase was acetonitrile-0.1 mol/L acetate buffer（pH 3.2）（50∶50,v/v）,and the flow rate was 0.8 mL/min.The detection wavelength was set at 299 nm.The method has good linearity in the ranges of 0.25-0.875 μg for cinobufagin（r=0.9990）,0.25-0.875 μg for resibufogenin（r=0.9991）,and 0.15-0.525 μg for bufalin（r=0.9990）.The average recoveries were 100.3%,100.0% and 98.0% for cinobufagin,resibufogenin,and bufalin,respectively.The results indicate that the method is simple,accurate,reproducible,and can be used for the quality control of bufogenins in toad venom.

关 键 词：反相高效液相色谱法 华蟾酥毒基 酯蟾毒配基 蟾毒灵 蟾酥 

分 类 号：O658[理学—分析化学]