小麦TaPK7基因的克隆及其在多种胁迫条件下的表达分析  

Cloning and Expression Analysis of TaPK7 under Different Abiotic Stresses in Wheat

作　　者：张洪映[1] 毛新国[2] 景蕊莲[2] 谢惠民[1] 昌小平[2] 

机构地区：[1]西北农林科技大学农学院,陕西杨陵712100 [2]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部作物种质资源与生物技术重点开放实验室,北京100081

出　　处：《麦类作物学报》2008年第2期177-182,共6页Journal of Triticeae Crops

基　　金：国家"863"项目(2006AA100201);国家"973"项目(2004CB117205)

摘　　要：为了揭示小麦TaPK7基因对不同逆境胁迫的应答机制,以SAPK7基因的全长cDNA序列为信息探针,采用电子克隆和RT-PCR的方法,从抗旱小麦品种旱选10号中克隆到一个包含1 074 bp开放阅读框、编码357个氨基酸的cDNA序列,命名为TaPK7。生物信息学分析表明,TaPK7同时具有磷酸化丝氨酸/苏氨酸和酪氨酸的活性。氨基酸序列比对发现,TaPK7与水稻、玉米、大麦等植物中受逆境胁迫诱导表达的直系同源基因高度同源。实时定量RT-PCR检测结果表明,TaPK7参与对高渗、高盐、低温等多种胁迫和ABA处理的应答反应,但在不同胁迫或处理下的表达模式不同,TaPK7对四种非生物胁迫的敏感性次序为:高盐>高渗>低温>ABA。Discovering and cloning genes related to drought tolerance are a foundation for improving the drought resistance of crop plants. It is reported that SAPK7 （AB125308） plays a crucial role un- der drought stress. In order to reveal the function of SAPKT-like gene in wheat, TaPK7 was isolated from wheat （Triticum aestivum L. ） based on SAPK7 sequence by in silico cloning and RT-PCR, which contains an open reading frame of 1 074 bp and encodes a protein of 357 amino acid. Sequence analysis indicated this putative protein possessed both activities of the serine/threonine and tyrosine kinases. Phylogenetic analysis results showed that TaPK7 was highly homologous to the orthologous genes from rice, maize and barley, which were also induced by abiotic stresses. Real-time quantitative PCR was introduced to investigate the expression pattern of TaPKT. The results revealed that TaPK7 was obviously responsive to the four abiotic stresses, but with a significant different expression patterns. The sensitivity degrees of TaPK7 responsing to stresses was in the order of high salinity〉hyperosmolality〉 low temperature （4℃）〉 abscisic acid.

关 键 词：小麦 TaPK7 克隆 非生物胁迫 

分 类 号：S512.1[农业科学—作物学] S330