小麦TaLEA4基因的克隆和表达  

Cloning and Characterizing of QX(Y15TaLEA4QX) Gene in Wheat

作　　者：王静英[1] 李永春[1] 王潇[1] 孟凡荣[2] 任江萍[1] 牛洪斌[1] 尹钧[1] 

机构地区：[1]河南农业大学／国家小麦工程技术研究中心,河南郑州 450002 [2]河南农业大学生命科学学院,河南郑州 450002

出　　处：《麦类作物学报》2008年第2期183-186,共4页Journal of Triticeae Crops

基　　金：河南省科技厅资助项目(0623011600)

摘　　要：为探讨小麦胚胎发育晚期丰富蛋白(LEA)在水分胁迫过程中的生物学功能,以PEG6000(20%)处理6.0 h的洛旱2号幼苗为材料,利用RT-PCR方法克隆了小麦的TaLEA4基因,并分析了该基因在水分胁迫过程中的表达特征。序列分析显示,TaLEA4基因编码的蛋白具有2个典型的LEA4保守域。半定量RT-PCR分析表明,在小麦幼苗根系中,随着PEG6000介导的水分胁迫时间的推移,该基因的表达量逐渐上升,24 h达到最强,48 h又迅速回落;而在小麦幼苗的叶片中,该基因在水分胁迫0.5 h的表达量较强,其他时间点的表达水平都较低。可见,TaLEA4基因与小麦水分胁迫反应密切相关,该基因在根系和叶片中的不同表达特性预示着该基因在根系和叶片的水分胁迫反应中发挥着不同的生物学功能。In order to study the function of Late Embryogenesis Abundant （LEA） proteins under water-stress in Wheat, seedlings of wheat cultivar ＂Luohan 2＂ treated with PEG6000 （20%） were pre pared and a gene TaLEA4 was cloned by RT-PCR, and its expression pattern was studied via SQ-RT-PCR. Analysis of the deduced amino acid of the gene showed that TaLEA4 protein contained two LEA4 conserved domains. SQ-RT-PCR analysis showed that the expression level of TaLEA4 in roots was gradually increased with the water-stress accumulation, and it reached the highest level at the time point of 24 hours after PEG treatment, however, the expression level declined at the time point of 48 hours after PEG treatment; The expression of the gene in leaves was strong at the time point of 0.5 hours after PEG treatment, but was relatively weak at other time points detected. These results showed that TaLEA4 gene was involved in the water-stress response of wheat, and the different expression patterns in roots and leaves indicated that this gene plays different roles in these two tissues under water-stress.

关 键 词：小麦 TaLEA4基因 克隆 表达 

分 类 号：S512.1[农业科学—作物学] S336