手性高效液相-圆二色检测器(HPLC-CD)分析葡萄糖的绝对构型  

A Method to Determine Absolute Configuration of Glucose by Chiral HPLC Using Circular Dichroism Detection

作　　者：宋爽[1] 王金辉[1] 孙铁民[1] 左文健[1] 周玉波[1] 

机构地区：[1]沈阳药科大学,辽宁沈阳110016

出　　处：《中国现代中药》2008年第3期13-15,共3页Modern Chinese Medicine

基　　金：新世纪优秀人才支持计划

摘　　要：目的:建立确定葡萄糖绝对构型的方法。方法:葡萄糖经衍生化引入对溴苯甲酰基发色团,从粗产物中分离出β型衍生物,再用配备圆二色(CD)检测器的手性高效液相进行分析。结果与结论:D型和L型葡萄糖的β型衍生物在手性高效液相上得到较好分离,通过β型衍生物的保留时间、色谱峰的方向(正峰或倒峰)以及在线扫描的圆二色谱图来确定葡萄糖的绝对构型。Objective：To develop a new method to determine absolute configuration of glucose. Methods：Glucose was derivatized with chromophores （p-bromobenzoate） , and β-derivates which had been separated from crude products were analyzed by chiral HPLC using circular dichroism detection. Results and Conclusion： β-derivates of D- glucose and L-glucose have a good revolution on the chiral HPLC, and the absolute configuration could be determined by retention time, direction of peak （positive or negative）, and circular dichroism spectra scanned on line.

关 键 词：葡萄糖 绝对构型 圆二色检测器 手性高效液相 

分 类 号：R971.1[医药卫生—药品] R969.1[医药卫生—药学]