HPLC测定仙鹤草秋芽、地下部分和地上部分鹤草酚的含量  

Study on HPLC Method for Determination of the Contents of Agrimophol from Different Parts of Agrimonia pilosa Ledeb.

作　　者：杜超[1] 李宁[1] 李铣[1] 孟大利[1] 张鹏[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《中国现代中药》2008年第3期16-18,共3页Modern Chinese Medicine

摘　　要：目的:建立HPLC测定仙鹤草秋芽、地下和地上部分鹤草酚含量的方法。方法:采用HPLC。色谱柱为DiamonsiL C_(18)柱(200mm×4.6mm,5μm),流动相为甲醇-水-三乙胺(58:42:0.2),流速为1.2mL·min^(-1),柱温为30℃,检测波长为325nm,进样量为20μL。结果:鹤草酚在0.011 97～0.112 5mg·mL^(-1)峰面积与对照品的质量浓度呈良好的线性关系,回归方程A=2.00×10~7p+2.89×10~3,r=0.999 8,平均回收率分别为101.1%、101.4%和101.4%,RSD分别为1.05%、1.08%和1.12%(n=9)。结论:该法简便、快捷、重现性好,为仙鹤草药材的质量控制提供了依据。Objective ： To obtain and develop an HPLC method for the determination of the contents of agrimophol from different parts of Agrirnonia pilosa Ledeb. Methods： The contents of agrimophol in Agrimonia pilosa Ledeb. was determined by HPLC with Diamonsil C18scolumn （250mm×4.6mm, 5μm） at 30℃. The mobile phase was methanol-water-triethylamine （58：42：0.2）with a flow rate at 1.20mL· min^-1 and the injection volume was 20μL The wavelength of the detector was set at 325nm. Results： The calibration curve was linear over the range of 0. 011 97 -0. 112 5mg·mL^-1 with correlation coefficient of 0. 999 8（n = 6）. Their average recoveries were 101.1%, 101.4% and 101.4 % respectively. The RSD values were 1.05 %, 1.08 % and 1.12 % （ n = 9 ） respectively. Conclusion： The method is sensitive, accurate,rapid,and it is suitable for the quality control of the medicinal material.

关 键 词：仙鹤草 鹤草酚 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R284.1