农杆菌介导几丁质酶基因对草莓转化条件的建立  

Establishment of Agrobacterium-mediated transformation system of strawberry with chitinase gene

作　　者：谢志兵[1] 钟晓红[1] 邓子牛[2] 

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128 [2]湖南农业大学细胞工程重点实验室

出　　处：《湖南农业大学学报（自然科学版）》2008年第1期25-28,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖北省教育厅项目(Q200526002)

摘　　要：为了得到优良的草莓抗病植株，采用根癌农杆菌介导法进行几丁质酶基因（chit42）对草莓叶片的转化，对转化过程中的一些因素进行研究，并得到了转化最优方案：乙酰丁香酮AS浓度80-120μmol／L，10-20min侵染，3d共培养．经PCR检测初步鉴定外源基因已经导入．With the transformation strawberry leaves, transgenic strawberry was obtained through the chit42 gene transformation by Agrobacterium tumefactions, so that disease-resistance transgenic plants were required. Factors on Agrobacterium tumifaciens-mediated gene transfer was discussed in details. We preliminarily determined an effective system for genetic transformation. The protocol was follows： AS 80-120 μtmol/L as co-culture medium, 10-20 minutes for infection, 3 days for co-culture. The regenerated kanamycin resistant plants were analyzed by PCR. The result indicated that the chit42 gene has been integrated into the strawberry.

关 键 词：草莓 几丁质酶基因 转化 

分 类 号：Q785[生物学—分子生物学]