HLA-DR、HLA-DQ分子在瘢痕疙瘩患者外周血单个核细胞中的变化  

Variation of HLA-DR、DQ molecule in the peripheral blood mononuclear cells of keloid patients

作　　者：孙东杰[1] 陈东明[1] 聂芳菲[1] 李生[1] 赵霞[1] 鲍卫汉[1] 

机构地区：[1]北京大学第三医院成形科,北京100083

出　　处：《中国美容医学》2008年第3期370-373,共4页Chinese Journal of Aesthetic Medicine

基　　金：国家自然科学基金资助项目(编号:30070778)

摘　　要：目的:探讨瘢痕疙瘩患者外周血单个核细胞(peripheral blood mononuclear cells,PBMC)HLA-DR和HLA-DQ分子的含量及其基因型的变化。方法:采用免疫细胞化学方法检测10例瘢痕疙瘩患者、10例扁平瘢痕患者及10例正常人外周血单个核细胞中HLA-DR、HLA-DQ分子的含量;应用聚合酶链反应-序列特异性引物(polymerase chain reaction-sequence specific primers,PCR-SSP)方法检测60例瘢痕疙瘩患者和110例正常人外周血单个核细胞HLA-DR和HLA-DQ的基因位点。结果:①瘢痕疙瘩患者外周血单个核细胞HLA-DR、HLA-DQ分子含量的变化:瘢痕疙瘩组HLA-DR+、HLA-DQ+细胞的积分光密度(813.16±62.77,420.12±94.25)高于扁平瘢痕组(636.22±133.95,302.77±89.77)和正常人组(597.88±166.36,308.57±44.05)(P<0.01);②瘢痕疙瘩患者外周血单个核细胞中HLA-DR和HLA-DQ基因位点的变化:瘢痕疙瘩组HLA-DR14和HLA-DQ5位点的抗原频率(0.16,0.28)明显高于对照组(0.06,0.15)(P<0.05),HLA-DR17和HLA-DQ8位点的抗原频率(0.02,0.03)明显低于对照组(0.13,0.15)(P<0.05)。结论:HLA-DR和HLA-DQ分子含量在瘢痕疙瘩患者外周血单个核细胞中增高提示瘢痕疙瘩患者机体可能处于高免疫应答状态;基因型检测结果则提示HLA-DR14、HLA-DQ5可能是瘢痕疙瘩的易感基因,HLA-DR17、HLA-DQ8可能是抵抗基因。Objective To investigate the variation of contents and genotypes of HLA-DR,DQ molecules in the peripheral blood mononuclear cells of keloid patients. Methods The expression levels and distribution patterns of HLA-DR,DQ in peripheral blood mononuclear cells were determined in 10 samples of keloid, 10 samples of thin and flat scar and 10 cases of normal people with immunocytochemical staining. The gene Iocuses of HLA-DR,DQ in peripheral blood mononuclear cells of 60 patients with keloid and 110 normal persons were detected by using polymerase chain reaction-sequence specific primers （PCR-SSP）. Results ①The variation of the HLA-DR,DQ molecules contents in the peripheral blood mononuclear cells of keloid patients： The integrated absorbance of HLA-DR^＋,HLA-DQ^＋ cells in the keloid group （813.16±62.77,420.12±94.25） was higher respectively than that of HLA-DR^＋, HLA-DQ^＋ cells in the flat scar （636.22± 133.95,302.77±89.77） and normal people groups （597.88± 166.36,308.57±44.05） （P〈0.01）. ②The variation of gene Iocuses of HLA-DR,DQ in the peripheral blood mononuclear cells of keloid patients： HLA-DR14 and HLA-DQ5 antigen frequencies were significantly higher in patients with keloid （0.16,0.28） than those in the controls （0.06, 0.15） （P〈 0.05）, while HLA-DR17 and HLA-DQ8 antigen frequencies were significantly lower in patients with keloid （0.02,0.03） than those in the controls （0.13,0.15） （P〈0.05）. Conclusion The contents increase of HLA-DR,DQ molecules in the peripheral blood mononuclear cells of keloid patients suggests that the organism of keloid patients may have strong immune reactions; The result of genotyping suggests that HLA-DR14 and HLA-DQ5 may be the predisposing genes of keloid while HLA-DR17 and HLA-DQ8 may be the counteracting genes of keloid.

关 键 词：瘢痕疙瘩 HLA-DR HLA-DQ 

分 类 号：R619.9[医药卫生—外科学]