石墨炉原子吸收光谱法测定尿中锰  

DETERMINATION OF MANGANESE IN URINE BY GRAPHITE FURNACE ATOMIC ABSORPTION SPECTROMETRY

作　　者：杨文英[1] 王宝旺[1] 籍术良[1] 

机构地区：[1]北京市通州区疾病预防控制中心,北京101100

出　　处：《现代预防医学》2008年第7期1324-1325,1330,共3页Modern Preventive Medicine

摘　　要：[目的]建立一种石墨炉原子吸收光谱法测定尿中锰的方法。[方法]尿样用硝酸溶液稀释后,直接用石墨炉原子吸收法测定。[结果]石墨炉原子吸收法测定尿中锰,灰化温度可达1200℃,原子化温度为2400℃。标准曲线的线性范围为0.00～20.00μg/L,方法的检出限为0.2μg/L。测定样品的相对标准偏差在2.1%～4.6%之间,回收率在90.9%～106.8%之间。分别用标准加入法和标准曲线法测定不同尿样中的锰,经统计学处理,两者测定结果无显著性差异。[结论]该方法操作简便、迅速,具有良好的精密度和准确度,可用于尿中锰的测定。[Objective] To establish a method for determination of manganese in urine by graphite furnace atomic absorption spectrometry （GFAAS）. [Methods] Manganese in urine was directly determined by GFAAS after the samples were diluted with nitric acid solution. [Results] In this method, the ashing temperature could reach 1200%. the atomization temperature was 2400℃. The linear range was from 0.00 to 20.00μg/L, the detection limit of the method was 0.2 μg/L. RSD of samples ranged from 2.1% to 4.6%. The average recoveries of samples were between 90.9%-106.8%. There were no significant differences between the results determined by standard curve method and standard addition method. [ Conclusion ] This method is simple, rapid, accurate and precise. It can be applied in the determination of manganese in urine.

关 键 词：石墨炉原子吸收光谱法 锰 尿 

分 类 号：R446.12[医药卫生—诊断学]