杀草强致甲状腺增生的量效关系初探  

EXPLORATION ON THE DOSE-EFFECT RELATIONSHIP BETWEEN AMITROLE AND THYROID HYPERPLASIA

作　　者：张素才[1] 周子人[1] 王文冬[1] 程薇波[1] 黄芹[2] 商维维[1] 

机构地区：[1]四川大学公共卫生学院病理教研室,成都610041 [2]华西附二院病理科

出　　处：《现代预防医学》2008年第7期1353-1354,1357,共3页Modern Preventive Medicine

摘　　要：[目的]研究杀草强(Amitrole)致甲状腺增生的量效关系,为杀草强的体内短期甄别甲状腺激素干扰物的标准化实验提供剂量设计依据。[方法]SD大鼠40只,随机分为1个对照组和4个实验组。对照组用蒸馏水灌胃,实验组用不同剂量的杀草强溶液灌胃。于实验期11d麻醉后股动脉放血处死动物,甲状腺组织称重后进行组织病理学观察及PAS染色观察。[结果]在100mg/(kg.day)剂量组,大鼠甲状腺脏器系数高于对照组,差异有统计学意义(P﹤0.05);在200mg/(kg.day)剂量组甲状腺增生以实心继发滤泡为主。[结论]杀草强可作为短期甄别甲状腺激素干扰物实验体系的验证药物,200mg/(kg.day)可作为验证实验的中心剂量。[Objective] To study the effect of amitrole on thyroid function in rats, and the dose-effect relationship in order to provide the data for the method of assessment for thyroid hormone disruptors in vivo in short-term with amitrole. [ Methods] 40 SD male rats were divided into live groups randomly （1 control group and 4 experimental groups）. Aruitrole was administered to experiment groups＇ rats by garage, about 50. 100. 200, 400 mg/kg/day respectively. The rats in the control group were given solvent by garage at the same volume. All animals were sacrificed one day after the last dosage. And then weighted their thyroid. The histological changes and the PAS stain of thyroid gland were observed. [ Results] Compared with the control group, the effect of thyroid gland increased significantly in 100 mg/kg/day group （P〈 0.05）; and in 200 mg/kg/ day group, the major histological changes in this group was secondary solid center follicle. [Conclusion] Selecting the amitrole as the verification drug of the short-term screening assay for thyroid hormone disruptors is feasible, and the 200 mg/kg/day may be the middle dose in the verilieation test.

关 键 词：杀草强 甲状腺激素干扰物 甲状腺 甄别实验 

分 类 号：R58[医药卫生—内分泌]