正常人肠道乳酸杆菌质粒谱与耐药谱的关系  

ANALYSIS ON THE RELATIONSHIP OF PLASMID PROFILE AND DRUG RESISTANT SPECTRUM OF LACTOBACILLUS FROM HEALTHY HUMAN

作　　者：熊鹿言[1] 陈欣[1] 邓秀英[1] 杨洋[1] 余倩[1] 

机构地区：[1]四川大学华西公共卫生学院医学检验教研室,成都610041

出　　处：《现代预防医学》2008年第7期1380-1382,1385,共4页Modern Preventive Medicine

摘　　要：[目的]了解正常人肠道乳酸杆菌对临床常用抗生素的耐受水平,研究质粒在乳酸杆菌中的存在频率,分析乳酸杆菌质粒与耐药性之间的关系。[方法]用微量肉汤稀释法及K-B纸片法测定正常人肠道乳酸杆菌对抗生素的耐药性;分析细菌的质粒图谱,进行质粒消除实验。[结果]正常人肠道乳酸杆菌对万古霉素的耐受性最强,对环丙沙星和左氟沙星的耐受性次之;对青霉素、氨苄西林完全敏感。40株菌株含有质粒,经消除质粒,乳酸杆菌对庆大霉素、四环素、头孢唑林、头孢呋辛、苯唑西林的耐受率明显降低,分别下降52.50%、50.00%、32.50%、52.50%、30.00%。[结论]正常人肠道乳酸杆菌对临床常用抗生素具有较强的耐受性,所有菌株都含有质粒,据质粒消除实验结果推测,乳酸杆菌质粒可能与其对庆大霉素、四环素、头孢呋辛、头孢唑啉和苯唑西林的耐受性有关。[ Objective ] To investigate the antibiotic resistance of intestinal lactobacillus in healthy human, the existent frequency of the plasmid in lactobacillus, to analyZe the relationship between the plasntid and the antibiotic resistance. [ Methods] Micro broth dilution techniques and K-B method were used to determine the antibiotic resistance of intestinal lactobacillus in healthy human. The plasmid profile of all strains was analyzed. [Results] The antibiotic resistance of human intestinal lacto, bacillus against Ciprofloxacin and Levofloxacin was strong and its resistance to Vancomycin was the strongest： It was absolutely sensitive to penicillin and ampicillin. 40 plasmids-crrying strains became less tolerant of gentamicin, tetracycline, cefazolin, cefuroxime and oxacillin after the elimination of plasimds, and their resistance rate decreased greatly by 52.50%, 50.00% , 32.50%, 52.50%, 30.00% respectively. [ Conclusion] The antibiotic resistance of human intestinal lactobacillus is very strong. All of strains contain plasmids. There might be a correlation between the plasmids and the resistance of strains to gentamycin, tetracycline, Cefazolin, cefazolin, and oxacillin.

关 键 词：质粒 耐药性 乳酸杆菌 正常人 

分 类 号：R378.2[医药卫生—病原生物学]