水通道蛋白1在人羊膜上皮细胞的表达及意义  

Expression of aquaporin1 in human amniotic membrane and its significance

作　　者：赵泽燕 漆洪波[2] 张华[2] 张建华[2] 

机构地区：[1]山西省运城市中心医院妇产科,运城044000 [2]重庆医科大学附属第一医院妇产科,重庆400016

出　　处：《重庆医科大学学报》2008年第2期163-165,共3页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助(30672244,30400616)

摘　　要：目的:探讨水通道蛋白1(Aquaporin1,AQP1)在人羊膜中的表达、定位及意义。方法:应用免疫组织化学方法检测12例正常足月妊娠产妇、11例羊水过少及9例羊水过多者足月妊娠产妇的羊膜组织中AQP1蛋白的表达。结果:AQP1在羊膜组织立方上皮细胞中有阳性表达,且主要位于细胞浆中,而在成纤维细胞中仅有微弱表达。羊水过少及过多患者与正常产妇的羊膜组织AQP1蛋白面积积分光密度值比较差异均有显著性(P<0.05)。结论:人羊膜组织表达AQP1蛋白,其在羊水的液体转运中可能起着重要作用,为羊水的水转运机制研究提供了分子生物学基础,并且为羊水量异常的治疗提供了新的思路。Objective：To investigate the expression, location and significance of aquaporinl （AQP1） in the tissue of amniotic membrane. Methods：Immunohistochemical method was used to examine the expression of aquaporinl in the tissue of amniotic membrane of 12 normal full-term pregnant women,11 full-term pregnamt woman with oligohydramnios and 9 with polyhydramnios. Result：Aquaporinl was expressed in cuboidal epithelial cells of amniotic membrane, and mainly in cytoplasm. There was but a little expression of aquaporinl in fibroblast of amniotic membrane tissue. There was statistically significant difference of the AQP1 expression between oligohydramnios,polyhydramnios and normal full-term pregnancy women integrative optical density in amniotic membrane tissue, respectively（P〈0.05）. Conclusion：AQP1 is expressed in cytoplasm of amniotic membrane tissue of human,and it may play a critical role in the process of water-transference. This study provides a molecular biological basis for the study the on molecular mechanism of amniotic fluid transport, and a new thinking for treating abnormal capacity for amniotic fluid.

关 键 词：水通道蛋白1(AQP1) 羊膜 

分 类 号：R714[医药卫生—妇产科学]