水稻单侧卷叶突变体B157遗传分析及基因初步定位  

Genetic Analysis and Mapping of the Unilateral Rolled Leaf Trait of Rice Mutant B157

作　　者：余东[1,2] 吴海滨[2,3] 杨文韬[1,2] 巩鹏涛[2] 李有志[1] 赵德刚[3] 

机构地区：[1]广西大学生命科学与技术学院,南宁530004 [2]海南省热带农业资源开发利用研究所,海南省农作物分子育种重点实验室,三亚572025 [3]贵州大学生命科学学院,贵州省农业生物工程重点实验室,贵阳550025

出　　处：《分子植物育种》2008年第2期220-226,共7页Molecular Plant Breeding

基　　金：国家科技支撑计划(2007BAD59B05);国家863项目(2006AA100101-2)资助

摘　　要：用60Co-γ射线诱变籼稻品种808,获得一个叶片单侧右向正面卷曲的突变体,命名为B157。以突变体B157与平展叶水稻808、日本晴(粳稻)、东洋超级稻(籼稻)杂交构建F2遗传群体。F2群体分离分析表明,该突变体的卷叶性状由一对隐性基因控制。利用东洋超级稻×B157构建的F2群体对该卷叶性状进行基因定位,初步将控制该卷叶性状的基因定位在水稻第1染色体上,并将其定位在RM272与RI02526两个分子标记之间,我们将该基因命名url1(t)(unilateral rolled leaf1)。定位分析表明,url1(t)基因与RM272与RI02526的遗传距离分别为0.6cM和2.0cM。进一步的insilico分析显示,url1(t)基因所在的两个标记间的物理距离为692.9kb,包含78个预测基因,其中有14个编码未知功能的表达蛋白,17个编码未知功能的假定蛋白,47个编码功能蛋白。基于TIGR数据库的分析发现,在这些预测基因中有三个预测基因与植物细胞分裂和生长有关,我们推测这三个预测基因可能与控制url1(t)卷叶性状有关。A rice unilateral rolled leaf mutant, named B157 （HITAR Accession No RH615）, was obtained in the rice mutant library derived from indica rice cultivar 808 radicated by ^60Co-7 ray, which has a obvious character of unilateral rolled leaf with the whole growth. In this paper we made three combinations to establish genetic populations for genetic analysis, the results confirmed that the mutant trait would be controlled by a single recessive gene, tentatively named as unilateral rolled leaf （urllt）. And then further mapped on the chromosome 1 with the flanking markers RM272 and RI02526, the gene might be in the 2.6 cM interval from 0.6 cM of RM272 and 2.0 cM of RI02526. Further in silico analysis suggested that the physical distance of the interval would be 692.9 kb, which includes 78 putative genes including 14 genes ecoding unknown function expression proteins, 17 genes encoding unknown function putative and 47 genes encoding function proteins based on the annotations in TIGR database. We did notice that three putative genes related to plant cell division and auxesis, which might make us link to the mutant trait of unilateral rolled leaf.

关 键 词：水稻 单侧内卷叶突变体 遗传分析 基因定位 

分 类 号：S511[农业科学—作物学]