源于长穗偃麦草的小麦新品系CH7034抗白粉病基因的染色体定位  

Chromosomal Location of Powdery Mildew Resistance Gene in Thinopyrum ponticum-derived Wheat Germplasm Line CH7034

作　　者：贺润丽[1] 畅志坚[1] 刘建霞[1] 詹海仙[1] 张晓军[2] 董春林[2] 

机构地区：[1]山西大学生物工程学院,太原030006 [2]山西省农业科学院作物遗传研究所,太原030031

出　　处：《分子植物育种》2008年第2期251-256,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(30671299;39870398);山西省自然科学基金(2006011093)资助

摘　　要：CH7034是一个兼抗小麦白粉病和条锈病的新种质材料,通过普通小麦与八倍体小偃麦"小偃7430"杂交、回交选育而成。为明确其白粉病抗性的遗传机制及抗性基因的染色体位置,用小麦高感品系"SY95-71"与CH7034杂交,所获F1、F2及其双亲在温室用白粉病E09菌系的15号小种接种,对CH7034的白粉病抗性进行鉴定和遗传分析。结果表明,无论是苗期还是成株期,CH7034对白粉病菌均表现为免疫,且具有与其抗性供体小偃7430及野生亲本长穗偃麦草相似的白粉病抗性,F1代抗病反应型为0或0'级,F2代抗感分离比符合R:S=3:1,说明CH7034抗性受显性单基因控制。用307对小麦微卫星引物对一个148株的F2群体进行分析,发现小麦微卫星标记Xgwm311与抗病基因连锁,遗传距离为12.4cM。用中国春缺—四体和双端体材料进一步验证与抗病相关的片段位于2A染色体的长臂上,进而将CH7034所含的抗白粉病基因定位于小麦的2AL上。Wheat germ plasm line CH7034, developed by backerossing a wheat-Thinopyrum ponticum partial amphiploid Xiaoyan7430 with susceptible wheat cultivars, wasfound to be resistant to both powdery mildew （Pro） and yellow rust. To determine the inheritance and chromosomal location of powdery mildew resistance gene, CH7034 was crossed with a susceptive wheat cultivar SY95-71. The seedling and adult tests of F1, F2 progeny and their parents were inoculated with Egt isolate E09 in greenhouse. F1 plants were consistent＇with their resistant parent CH7034 and resistant to Pro. In the F2 populations, the observed phenotypic classes for powdery mildew reaction satisfactorily fitted a 3R： 1S segregation ratio, suggesting that the powdery mildew resistance in CH7034 be genetically controlled by a single dominant gene. Using bulked segregation analysis （BSA）, a total of 148 F2 plants from the cross CH7034（R）/SY95-71 （S） was screened with 307 wheat microsatellite （SSR） primer pairs to detect molecular markers linked to Pm resistance gene. The microsatellite marker linked to Pm resistance gene position were conformed using Chinese Spring nullitetrasomic and ditelosomic lines. The results indicated that the Pm resistance gene present in CH7034 might be linked to microsatellite marker Xgwm311-2A with the genetic distance of 12.4 eM and mapped on chromosome 2AL.

关 键 词：普通小麦 长穗偃麦草 白粉病抗性 基因定位 

分 类 号：S512.1[农业科学—作物学] S435.121.4