用SSR标记初步分析高州普通野生稻的籼粳分化  

Preliminary Analysis of the Indica-Japonica Differentiation among Natural Wild Rice Populations in Gaozhou by SSR Markers

作　　者：陈雨[1] 杨庆文[2] 潘大建[1] 曲延英[3] 范芝兰[1] 陈建酉[1] 李晨[1] 

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]中国农业科学院作物科学研究所,北京100081 [3]广东省农业科学院水稻研究所,广州510640

出　　处：《分子植物育种》2008年第2期263-267,共5页Molecular Plant Breeding

基　　金：广东省科技基础条件项目(2006B60101021);国家科技支撑计划(2006BAD13B01-11);科研院所社会公益研究专项(2004DIB3J090)资助

摘　　要：利用34对SSR引物对高州普通野生稻(简称"高野",下同)7个自然居群217份个体进行研究,并结合统计软件进行遗传距离和遗传结构分析。通过已明晰籼、粳类型的中国栽培稻微核心种质作对照检测,所用引物能有效区分籼粳亚种,并在高野材料中扩增出籼稻带型、粳稻带型以及野生稻特殊带型。结果表明,高野群体总体比较原始,已经出现了籼粳分化,其中偏粳多于偏籼,但这种分化是初步的。本研究为进一步明确高野在中国栽培稻的起源演化上的地位、并对其有效的发掘利用提供科学参考。The lndica-Japonica differentiation of 217 individuals from the 7 natural population of Oryza ruffpogon in Gaozhou was analyzed using 34 pairs of SSR primers. The SSR primers used in this research could identify lndica and Japonica types of cultivated dee （Oryza sativa）, and could amplify Japonica-like type, lndica-like type, wild dee-special type among the Oryza rufipogon in Gaozhou. Although it was homogenetic in gene structure, lndica-Japonica differentiation have presented to natural population of Oryza rufipogon in Gaozhou, there into the number of lndica-like type was more than Japonica-like type, and the differentiation was elementary. This work help to know the status of Oryza rufipogon in Gaozhou on origin and evolvement of cultived rice in China, and also offer referrence to use it effectivly.

关 键 词：普通野生稻 籼粳分化 微卫星标记 

分 类 号：S511.9[农业科学—作物学] S511.03