大规模高通量方法在蛋白质相互作用研究中的应用  

The Applications of Large-scale High-throughput Methods for Studying Protein-protein Interaction

作　　者：符庆瑛[1] 高钰琪[1] 

机构地区：[1]第三军医大学高原军事医学系病理生理学与高原生理学教研室,重庆400038

出　　处：《生物化学与生物物理进展》2008年第3期246-254,共9页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金"高原低氧高寒的损伤与适应机制的研究"重大项目(30393131)~~

摘　　要：随着后基因组时代的到来,阐明蛋白质间相互作用关系成为蛋白质研究的又一热点,促进了相关技术的不断产生、发展和完善.其中涉及到诸多大规模高通量的方法,如双杂交系统、噬菌体展示、质谱、蛋白质芯片以及生物信息学等,这为系统分析蛋白质相互作用提供视点,有望在蛋白质组学研究中发挥重要作用.每种方法各有其优缺点且适用范围不同,在一定程度上各方法的实验结果互为补充.现拟就这些大规模高通量方法的研究进展及其在蛋白质相互作用研究中的应用作一综述.With the advent of post-genomic era, identification of protein-protein interaction has become another hot spot of protein research and promoted the invention, development and complement of relative techniques. Major large-scale high-throughput methods, such as two-hybrid system, bacteriophage display, mass spectrometry, protein microchips and bioinformatics have provided a global view of protein-protein interaction, and are hopeful to play an important role in proteomics. Each method may have its specific strengths and weaknesses as well as differences in coverage, to some extent, the data based on these methods can complete each other. Here, recent progress of these large-scale high-throughput methods and their applications for studying protein-protein interaction are reviewed.

关 键 词：蛋白质相互作用 大规模 高通量筛选 

分 类 号：Q51[生物学—生物化学] Q503