蛋白酪氨酸磷酸酶-1B基因多态性与2型糖尿病的相关性研究  

Study on Relations between Protein Tyrosine Phosphastase-1B Gene Polymorphism and Type Ⅱ Diabetes Mellitus

作　　者：冯秋霞[1] 程丽静[2] 芦露[1] 罗兰[1] 高政南[1] 

机构地区：[1]辽宁省大连市中心医院内分泌科,116011 [2]大连医科大学附属第二医院肾内科,116027

出　　处：《中国误诊学杂志》2008年第10期2272-2273,共2页Chinese Journal of Misdiagnostics

基　　金：国家自然科学基金(编号:30471926;30671110);科技部973课题(编号:2006CB503900)资助

摘　　要：目的:探讨大连地区2型糖尿病(T 2DM)人群及家系中蛋白酪氨酸磷酸酶-1B(PTP-1B)基因981位点多态性与糖尿病的相关性。方法:采用人群病例对照研究,病例组101例,对照组309例;采用家系研究,选取T 2DM家系的T 2DM患者、非糖尿病一级亲属、健康配偶各80例。应用聚合酶链反应(PCR)及限制性片段长度多态性(RFLP)方法,检测各组PTP-1B基因981位点基因型及其频率。结果:人群病例对照研究和家系研究中,各组PTP-1B基因均以CC基因型为主,C等位基因频率差异无显著性。结论:大连地区T 2DM的人群病例对照研究及家系研究中,未发现PTP-1B基因的981位点C→T多态性与T 2DM的发病有关。Objective： To investigate the polymorphism of 981 point in protein tyrosine phosphastase-lB （PTP- 1B） gene of the patients with type Ⅱ diabetes mellitus （T2DM） in Dalian. Methods ：101 patients with T2DM and 309 healthy subjects were selected in population case-control research. There were 80 families in family study. Different distribution in genotypes of all groups was analyzed. Results ：The major genotype of all groups in population case-control study and family study was homozygote CC genotype. The frequencies of C alleles in all groups were similar. Conclusion. The polymorphism of 981 point in PTP-1B gene is not associated with the risk of type Ⅱ diabetes mellitus in Dalian.

关 键 词：糖尿病 Ⅱ型/遗传学 蛋白酪氨酸磷酸酶/遗传学 基因 多态现象(遗传学) 人类 

分 类 号：R587.105.34[医药卫生—内分泌]