龙芽葱木总皂苷对大鼠早期糖尿病心肌病的保护作用及机制  

Protective effect of total arasolides of aralia elata(Miq) seem on cardiomyopathy in rats with early stage diabetes and its mechanism

作　　者：奚树刚[1] 张学新[2] 张文杰[2] 魏征人[3] 赵春燕[2] 

机构地区：[1]吉林大学第一医院内分泌科,吉林长春130021 [2]吉林大学基础医学院生理学教研室,吉林长春130021 [3]吉林大学基础医学院药理学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2008年第2期209-213,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅资助课题(20050922)

摘　　要：目的:探讨龙芽葱木总皂苷(TASAES)对大鼠早期糖尿病心功能的影响和心肌结构保护作用及其机制。方法:应用链脲佐菌素(STZ)复制大鼠糖尿病(DM)模型,实验设正常对照组(C组)、模型组(M组)及TASAES4.9、9.8、19.6mg.kg-1组(T1,T2,T3组)。建模8周时测定各组大鼠血流动力学参数,电子显微镜观察超微结构变化,RT-PCR技术检测结缔组织生长因子(CTGF)在核酸水平上的转录情况。结果:T2、T3组大鼠左室收缩压(LVSP)、等容收缩期左室内压上升(下降)的最大速率(±dp/dtmax)的绝对值明显高于M组(P<0.05或P<0.01);电镜显示T1、T2及T3组大鼠心肌肌丝排列致密,肌节明暗带结构清晰,其中T2、T3组心肌超微结构改善效果较为显著;RT-PCR结果显示,CTGF在T2、T3组的表达显著低于在M组大鼠心肌的表达(P<0.01)。结论:TASAES可提高DM大鼠的心功能,且呈剂量依赖性关系;对心肌的保护作用机制可能与抑制CTGF在DM大鼠心肌的表达及提高大鼠心功能有关。Objective To discuss the effect of total arasolides of aralia elata （Miq） seem （TASAES） on cardiac function and protective effect on mgocardial structure in rats with early stage diabetes. Methods The models of diabetic rats were made by an intra-abdominal injection of streptozotocin （STZ）. Rats were divided into control group （C group）, model group （M group） and TASAES 4.9, 9.8, 19.6 mg·kg^-1 groups （T1, T2, T3 groups）. At the time of 8 weeks, the hemodynamic parameters were detected in various groups; uhrastructure of cardiomyocytes was observed under electron microscope; the expression of connective tissue growth factor （CTGF） was detected by RT-PCR technique at the molecular level. Results Compared with model group, TASAES increased the absolute value of left ventricular systolic pressure （LVSP） and ±dp/dtmax in diabetic rats, especially in high and middle dosage groups （P〈0. 05, P〈0. 01）, it improved the uhrastructure of cardiomyocytes and reduced the expression of CTGF in high and middle dosage groups （P〈0. 01）. Conclusion TASAES can improve the cardiac function of diabetic rats in a dose dependent manner, and its protective effect may be related to the reduction of CTGF and enhancement of cardiac contractility.

关 键 词：糖尿病心肌病 血流动力学 结缔组织生长因子 心肌结构 

分 类 号：R285.5[医药卫生—中药学] R587.1[医药卫生—中医学]