高糖状态下肾脏系膜细胞葡萄糖转运蛋白1的表达及氟伐他汀的干预研究  

Effect of fluvastatin on GluT-1 expression in glomerular mesangial cells exposed to high glucose

作　　者：李英[1] 段惠军[2] 张丽红[1] 张涛[1] 张进贵[1] 史永红[2] 林海英[1] 

机构地区：[1]河北医科大学第三医院肾内科,石家庄050051 [2]河北医科大学病理学教研室

出　　处：《中国糖尿病杂志》2008年第3期180-183,共4页Chinese Journal of Diabetes

摘　　要：目的观察高糖环境中大鼠肾脏系膜细胞(MC)葡萄糖转运蛋白1(GluT-1)及转化生长因子1(TGF-β1)mRNA表达变化以及氟伐他汀(Flu)的干预作用,探讨其保护肾脏的可能机制。方法原代培养MC分为正常对照(NG)组、甘露醇(MG)组、高糖(HG)组及HG+Flu组,RT-PCR法检测细胞中GluT-1 mRNA和TGF-β1 mRNA的表达,放免法测定各组细胞培养上清液中Ⅳ型胶原含量。结果HG组各时相点GluT-1 mRNA、TGF-β1 mRNA表达量均高于NC组(P<0·01),从48h开始HG组细胞上清液Ⅳ型胶原的含量较NC组增高(P<0·05),而HG+Flu组GluT-1 mRNA表达量、TGF-β1 mRNA的表达、细胞上清液Ⅳ型胶原含量均较同时点HG组明显降低(P<0·01)。结论高糖刺激使MC内GluT-1 mRNA表达增高,TGF-β1 mRNA表达上调,Ⅳ型胶原分泌增多。高糖状态下,Flu抑制细胞外基质积聚的作用可能与其抑制高糖引起MCGluT-1过表达、减少TGF-β1的合成有关。Objective To investigate the effect of fluvastatin on the expressions of GluT-1 and TGF- β1 mRNA in glomerular mesangial cells（GMC） exposed to high glucose,discuss the role of GluT1 in the onset and progression of diabetic nepropathy,and explore the possible mechanism for fluvastatin to protect renal function. Methods GMC was cultured at high glucose concentration with （HG＋ flu group） or without （HG group） fluVastatin. RT-PCR was used to detect GluT1 and TGF-β1 mRNA expressions, and radioimmunoassay for the content of type Ⅳ collagen in the supernatant. Results The expressions of GluT1 and TGF-β1 mRNA and the content of type Ⅳ collagen in the supernatant in GMC culture was higher in HG group than in normal glucose group. The above parameters were significantly lower in HG＋ flu group than in HG group. Conclusions Under high glucose stimulation, the up-regulated expression of Glu T-1 mRNA is able to induce the increased expression of TGF-β1 mRNA and the increased synthesis and secretion of type Ⅳ collagen in glomerular mesangial cells.

关 键 词：糖尿病肾病 肾小球系膜细胞 葡萄糖转运蛋白1 氟伐他汀 

分 类 号：R587.2[医药卫生—内分泌]