50 cGyγ射线照射人肝细胞基因差异表达谱研究  

STUDY OF DIFFERENTIAL GENE EXPRESSION IN HUMAN HEPATOCYTE EXPOSED TO 50 cGy γ RAY

作　　者：闻建华[1] 李建国[1] 田还成[1] 李燕玲[1] 王晓莉[1] 左雅慧[1] 

机构地区：[1]中国辐射防护研究院放射医学与环境医学研究所,太原030006

出　　处：《辐射防护》2008年第2期84-89,共6页Radiation Protection

基　　金：国防"十一五基础科研"资助项目(项目编号:012060201)

摘　　要：本研究利用基因芯片技术对50 cGyγ射线照射的人肝细胞和未照射的人肝细胞基因差异表达谱进行了研究和分析。研究结果表明:在所分析的14 000多条基因中,共有614条差异基因表达谱,其中明显上调的差异表达基因有521条,明显下调的差异表达基因93条。主要涉及到以下几种类型基因:与线粒体调控有关的基因;与甲型肝炎病毒受体有关的基因;与肿瘤坏死因子有关的基因;与细胞周期调控有关的基因;与激酶有关的基因;以及与锌指蛋白有关的基因等等。用RT-PCR进一步验证了部分差异表达基因:四个表达上调基因HAVcr-1、HAVcr-2、MFTC、MOAP1和2个表达下调基因TRIP12、DCN,检测结果与基因芯片结果相一致。The study analyzed the differential transcriptional profile of the normal human hepatic cell and the human hepatic cell radiated with 50 cGy γ ray by gene chip technique. The results showed that there were 614 differentially expressed genes among 14 112 human genes analyzed, in which 521 genes were up-regulated and 93 genes down-regulated. These genes are associated with mitochondrial regulation, homo sapiens hepatitis A virus cellular receptor, tumor necrosis factor, cell cycle regulation, kinase and zinc finger protein etc. RT-PCR results indicated that up-regulated expression of gene HAVcr-1, HAVcr-2, MFTC MOAP1 and down-regulated expression of gene TRIP12, DCN were consistent with gene chip data.

关 键 词：Γ射线 人 肝细胞 基因芯片 差异表达基因 

分 类 号：R144[医药卫生—公共卫生与预防医学]