利多卡因对家兔心肌缺血/再灌注损伤Na+-K+-ATPase、Ca^(2+)-Mg^(2+)-ATPase及NO的作用  

Effect of lidocaine on myocardial Na+-K+-ATPase,Ca^(2+)-Mg^(2+)-ATPase and serum NO during myocardial ischemia/reperfusion injury in rabbits

作　　者：雍文成 冷玉芳[2] 石慧文[3] 

机构地区：[1]西安市结核病医院麻醉科,陕西西安710068 [2]兰州大学第一医院麻醉科,甘肃兰州730050 [3]陕西省人民医院麻醉科,陕西西安710068

出　　处：《现代医药卫生》2008年第8期1107-1108,共2页Journal of Modern Medicine & Health

摘　　要：目的:研究利多卡因对心肌缺血/再灌注损伤家兔心肌Na+-K+-ATPase、Ca2+-Mg2+-ATPase及血清NO的影响。方法:24只家兔随机分为3组:假手术组(A组)、缺血/再灌注组(B组)、利多卡因组(C组),每组8只。B组、C组阻断左冠状动脉前降支40 min,再灌注90 min,A组仅穿线不结扎。C组于开放冠状动脉再灌注前经颈静脉注射利多卡因5 mg/kg,A、B组注射等量生理盐水。于再灌注90min取颈静脉血测定NO。再灌注90 min后取心肌组织测定NOS、Na+-K+-ATPase、Ca2+-Mg2+-ATPase。结果:心肌组织Ca2+-Mg2+-ATPaseB组较A、C组降低(P<0.01);Na+-K+-ATPase B组较A、C组降低(P<0.01);血清NO、心肌组织NOS:B组较A、C组降低(P<0.01)。结论:利多卡因对心肌缺血/再灌注损伤具有保护作用。Objective ：To study the effect of lidocaine on myocardial Na^＋ -K^＋ -ATPase ,Ca^2＋ -Mg^2＋ -ATPase and serum NO during myocardial iscbemia/reperfusion injury in rabbits. Methods：24 rabbits were randomly divided into three groups,that is,sham operation group（group A）,iscbemia/reperfusion injury group（group B）,hdocaine group（group C）,with 8 rabbits in each group. The left coronary artery anterior desending branch was blocked in groups B and C for 40 mlnutes,and then the reperfusion was performed for 90 minutes,while group A only braided and not being ligated.The jugular vein in group C was injected with lidocaine 5 mg/kg before reperfusion ,while the groups B and A were injected with equivalent physiological saline.After reperfusion,the jugular blood was drawn to determine NO,while the myocardial tissue was taken to determine NOS, Na^＋ -K^＋ -ATPase and Ca^2＋ -Mg^2＋ -ATPase.Results：The myocardial tissue Ca^2＋ -Mg^2＋ -ATPase in group B was lower than that in groups A and C （P〈0.01）;the serum NO and myocardial tissue Na^＋ -K^＋ -ATPase and NOS in group B were lower than those in groups A and C （all P〈0.01）. Conelusion：Lidocaine plays a protective role on cardiac muscle during myocardial ischemia/reperfusion injury.

关 键 词：利多卡因 心肌缺血/再灌注损伤 Ca2^+ - MG^2+ —ATP酶 Na^+ -K^+ -ATP酶 一氧化氮 一氧化氮合酶 

分 类 号：R614[医药卫生—麻醉学]