杜檊氏菌B2的蓝色素成分的分离及化学结构解析  

Separation, purification and structure identification of purple pigments from Duganella B2

作　　者：王海胜[1] 卢元[2] 薛园[2] 阮志勇[1] 姜瑞波[1] 邢新会[2] 娄恺[3] 

机构地区：[1]中国农业科学院农业资源与农业区划研究所,北京100081 [2]清华大学化学工程系,北京100084 [3]新疆农业科学院微生物应用研究所,新疆乌鲁木齐830000

出　　处：《化工学报》2008年第3期630-635,共6页CIESC Journal

基　　金：国家微生物菌种资源科技基础条件平台项目(2005DKA21201);国家自然科学基金项目(20676071)~~

摘　　要：A bacterium B2 isolated from the Tianshan glacier of Xinjiang could produce blue pigments.According to 16S rDNA analysis, this isolate belonged to Duganella Genus .Two compounds were separated and purified from the cultivated Duganella B2 , named Blue-Ⅰand Blue-Ⅱ, respectively.From the spectra data of UV, MS and NMR of the compounds, Blue-Ⅰwas confirmed to be deoxyviolacein and Blue-Ⅱ was violacein.Blue-Ⅰand Blue-Ⅱ had the respective molecular weights of 327.2 and 343.2,and showed the characteristic absorption peaks at the respective 560 and 572 nm within the visible light range in ethanol solution.These results will be useful for developing the bioprocess for producing bacterial violacein.A bacterium B2 isolated from the Tianshan glacier of Xinjiang could produce blue pigments. According to 16S rDNA analysis, this isolate belonged to Duganella Genus . Two compounds were separated and purified from the cultivated Duganella B2 , named Blue-Ⅰ and Blue-Ⅱ, respectively. From the spectra data of UV, MS and NMR of the compounds, Blue-Ⅰ was confirmed to be deoxyviolacein and Blue- Ⅱ was violacein. Blue-Ⅰ and Blue-Ⅱ had the respective molecular weights of 327.2 and 343.2, and showed the characteristic absorption peaks at the respective 560 and 572 nm within the visible light range in ethanol solution. These results will be useful for developing the bioprocess for producing bacterial violacein.

关 键 词：结构鉴定 杜擀氏菌属 紫色杆菌素 脱氧紫色杆菌素 

分 类 号：R93[医药卫生—生药学] TQ62[医药卫生—药学]